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CBL154

Sigma-Aldrich

Anti-H-CAM Antibody, clone F10-44-2

clone F10-44-2, Chemicon®, from mouse

Synonym(s):

CD44

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

F10-44-2, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable

isotype

IgG2a

NCBI accession no.

NM_000610.3
NM_001001389.1
NM_001001390.1
NM_001001391.1
NM_001001392.1

UniProt accession no.

P16070

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD44(960)

Related Categories

Application

Identification of CD44 positive cells by flow cytometry and immunocytochemistry, in particular with formalin fixed paraffin wax embedded sections of lymphoid and epithelial tissues (high temperature antigen retrieval in 10mM citrate buffer, pH6.0, is recommended).

Studies involving T, B cell, monocyte, granulocyte T and B cell binding to HEV during lymphocyte circulation and movement of leucocytes to inflammatory sites.

Studies on HCAM (homing receptor) function.

Immunoprecipitation.

Optimal working dilutions must be determined by the end user.
This Anti-H-CAM Antibody, clone F10-44-2 is validated for use in FC, IP, IH, IH(P) for the detection of H-CAM.

Physical form

Format: Purified

Analysis Note

Control
POSITIVE CONTROL: Tonsil; stains all T-cells in the paracortex

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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José M Díez et al.
Stem cell research & therapy, 6, 28-28 (2015-04-19)
Fetal bovine serum (FBS) is an animal product used as a medium supplement. The animal origin of FBS is a concern if cultured stem cells are to be utilized for human cell therapy. Therefore, a substitute for FBS is desirable.
Larissa M Macedo et al.
Journal of cellular physiology, 236(1), 366-378 (2020-06-11)
The effects of the renin-angiotensin system (RAS) on stem cells isolated from human dental apical papilla (SCAPs) are completely unknown. Therefore, the aim of this study was to identify RAS components expressed in SCAPs and the effects of angiotensin (Ang)
Arantxa Blázquez-Prunera et al.
Stem cell research & therapy, 8(1), 103-103 (2017-04-30)
Mesenchymal stem cells (MSCs) show promising characteristics for their use in advanced therapy medicinal products. However, there are some unresolved concerns, such as the use of animal components for their expansion. In this study we assessed the suitability of a
Giulia Detela et al.
Biotechnology journal, 13(2) (2018-01-16)
Human mesenchymal stromal cells (hMSCs) are excellent candidates for cell therapy but their expansion to desired clinical quantities can be compromised by ex vivo processing, due to differences between donor material and process variation. The aim of this article is

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