345788

β-Galactosidase, E. coli

• Synonym(s): β-Galactosidase, E. coli, β-D-Galactoside galactohydrolase
• CAS Number: 9031-11-2
• Enzyme Commission number: 3.2.1.23 (BRENDA, IUBMB)
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: Escherichia coli
• Quality Level: 200
• form: lyophilized solid
• specific activity: ≥600 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: water: 2%
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native E. coli β-galactosidase. Inducible enzyme not found in eukaryotic cells that is used as a reporter gene. Used to assess efficiency of transfection. Also used in ELISA and immunocytochemical techniques. Histochemical substrates include X-Gal (Cat. No. 203782).

Native E. coli β-galactosidase. Inducible enzyme not found in eukaryotic cells that is used as a reporter gene. Used to assess efficiency of transfection. Also used in ELISA and immunocytochemical techniques. Histochemical substrates include X-Gal (Cat. No. 203782).

Packaging

Yes

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of p-nitrophenyl-β-galactoside per min at 37°C.

Other Notes

Zeleny, R., et al. 1997. Anal. Biochem.246, 96.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable