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HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18

HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

581324-U

Ascentis® C18 HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

standard

Product No.
Description
Pricing

47763

Cholecalciferol (D3)

analytical standard

47768

Ergocalciferol (D2)

analytical standard

CONDITIONS

column

Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)

mobile phase

acetonitrile

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 290 nm

sample

50 μg/mL each in acetonitrile

Description

Analysis Note

This application demonstrates the suitability of the Ascentis C18 for the analysis of the fat-soluble vitamins D2 and D3 . Structures along with the optimized chromatogram obtained on the Ascentis C18 are presented.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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