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  • Characteristics of organic acids in the fruit of different pumpkin species.

Characteristics of organic acids in the fruit of different pumpkin species.

Food chemistry (2013-11-23)
Agnieszka Nawirska-Olszańska, Anita Biesiada, Anna Sokół-Łętowska, Alicja Z Kucharska
ABSTRACT

The aim of the research was to determine the composition of organic acids in fruit of different cultivars of three pumpkin species. The amount of acids immediately after fruit harvest and after 3 months of storage was compared. The content of organic acids in the examined pumpkin cultivars was assayed using the method of high performance liquid chromatography (HPLC). Three organic acids (citric acid, malic acid, and fumaric acid) were identified in the cultivars, whose content considerably varied depending on a cultivar. Three-month storage resulted in decreased content of the acids in the case of cultivars belonging to Cucurbita maxima and Cucurbita pepo species, while a slight increase was recorded for Cucurbita moschata species.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
L-(−)-Malic acid disodium salt, ≥95% (titration)
Supelco
Citric acid, Anhydrous, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
DL-Malic acid, 99%
Sigma-Aldrich
Citric acid, SAJ first grade, ≥99.5%
USP
Citric acid, United States Pharmacopeia (USP) Reference Standard
Sigma-Aldrich
Citric acid, BioUltra, anhydrous, ≥99.5% (T)
Sigma-Aldrich
D-(+)-Malic acid, unnatural form, ≥97.0% (T)
Sigma-Aldrich
DL-Malic acid, ReagentPlus®, ≥99%
Sigma-Aldrich
Fumaric acid, FCC, FG
Sigma-Aldrich
Fumaric acid, ≥99.0% (T)
Sigma-Aldrich
Citric acid, ACS reagent, ≥99.5%
Sigma-Aldrich
Citric acid, ≥99.5%, FCC, FG
Sigma-Aldrich
DL-Malic acid, meets analytical specification of FCC, E296, 99-100.5% (alkalimetric)
Sigma-Aldrich
Citric acid, 99%
Sigma-Aldrich
Malic acid, meets USP/NF testing specifications
Fumaric acid, European Pharmacopoeia (EP) Reference Standard
Citric acid, anhydrous, European Pharmacopoeia (EP) Reference Standard
Supelco
Fumaric acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
DL-Malic acid, ≥98% (capillary GC)
Supelco
Citric acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
Citric acid, anhydrous, suitable for cell culture, suitable for plant cell culture
Supelco
D-Malic acid, analytical standard
Supelco
Malic acid, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Citric acid, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥99.5%
Supelco
Fumaric Acid, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Citric acid, meets analytical specification of Ph. Eur., BP, USP, E330, anhydrous, 99.5-100.5% (based on anhydrous substance)
Supelco
DL-Malic acid, analytical standard