MAK501

Glucose Oxidase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• Synonym(s): Glucose Oxidase Assay Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 100
• application(s): food and beverages; pharmaceutical
• detection method: colorimetric; fluorometric
• relevant disease(s): endocrinological disorders, diabetes
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Glucose oxidase catalyzes the oxidation of glucose from D-glucose to D-glucono-δ-lactone. Physiologically, it aids in the breakdown of glucose into smaller metabolites. It is widely used in electrochemical glucose sensors designed for diabetes patients.

Features and Benefits

• Linear detection range: 0.02 to 10 U/L glucose oxidase for the colorimetric assay and 0.002 to 1.5 U/L for the fluorometric assay.

Suitability

The kit is suitable for determining glucose oxidase activity in cell lysate, culture medium and other biological samples, as well as for studying the effects of drugs on glucose metabolism.

Principle

The Glucose Oxidase Assay Kit uses a single working reagent that combines the glucose oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to glucose oxidase activity in the sample.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2