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Supelco

Ascentis® Express 90 Å RP-Amide (2.7 μm) HPLC Columns

L × I.D. 5 mm × 4.6 mm, HPLC Guard Cartridge, pkg of 3 ea

Synonym(s):

Core-shell (SPP) Fused Core polar embedded HPLC column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® Express RP-Amide, 2.7 μm Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 4.6 mm, pkg of 3 ea

material

stainless steel column

Quality Level

Agency

suitable for USP L60

product line

Ascentis®

feature

endcapped

packaging

pkg of 3 ea

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

5 mm × 4.6 mm

surface area

135 m2/g

matrix

Fused-Core® particle platform
superficially porous particle

matrix active group

amide, alkyl phase

particle size

2.7 μm

pore size

90 Å

operating pH

2-9

application(s)

food and beverages

separation technique

reversed phase

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General description

Ascentis Express Guard Columns provide physical (filtration) and chemical protection for costly analytical columns without compromising the very high performance of Ascentis Express columns. These Ascentis Express guard columns are capable of continuous use at pressures up to 9000 psi (600 bar) with only hand-tightening. Guard cartridges are easily replaced without removing the guard column holder from the flow path. The cartridges are packed with Ascentis Express Fused-Core® particles. Order guard column holder separately.

Application


  • Polycaprolactone Composite Micro/Nanofibrous Material as an Alternative to Restricted Access Media for Direct Extraction and Separation of Non-Steroidal Anti-Inflammatory Drugs from Human Serum Using Column-Switching Chromatography: This research utilized the Ascentis Express RP-Amide, demonstrating its effectiveness in column-switching chromatography for the extraction and separation of non-steroidal anti-inflammatory drugs directly from human serum. The study highlights the column′s utility in handling complex biological matrices, an essential aspect for pharmacokinetic and drug monitoring studies (Raabova et al., 2021).

  • An HPLC-MS method for the quantification of new acetylcholinesterase inhibitor PC 48 (7-MEOTA-donepezil like compound) in rat plasma: Application to a pharmacokinetic study: This paper details the use of the Ascentis Express RP-Amide in a validated HPLC-MS method for the analysis of PC 48, a novel acetylcholinesterase inhibitor, in rat plasma. The method′s application to pharmacokinetic studies underscores its significance in the development and therapeutic monitoring of new drugs (Mzik et al., 2016).

  • On-line SPE-UHPLC method using fused core columns for extraction and separation of nine illegal dyes in chilli-containing spices: Demonstrating the versatility of the Ascentis Express RP-Amide, this study employed the column in an on-line solid-phase extraction (SPE) coupled with UHPLC to detect illegal dyes in food samples. The method′s robustness and high-throughput capabilities make it ideal for ensuring food safety and compliance with regulatory standards (Khalikova et al., 2014).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Martin Mzik et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1020, 85-89 (2016-04-01)
A simple, rapid and sensitive method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed and validated for the quantitative determination in rat plasma of a new candidate for AD treatment, namely PC 48 (a 7-MEOTA-donepezil like compound) in
Erzsébet Oláh et al.
Journal of chromatography. A, 1217(23), 3642-3653 (2010-04-23)
Today sub-2 microm packed columns are very popular to conduct fast chromatographic separations. The mass-transfer resistance depends on the particle size but some practical limits exist not to reach the theoretically expected plate height and mass-transfer resistance. Another approach applies
S Fan et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 30(12), 2093-2101 (2013-10-22)
A liquid chromatography-linear ion-trap spectrometry (LC-MS³) method using β-receptor molecular-imprinted polymer (MIP) solid-phase extraction (SPE) as clean-up was developed to determine simultaneously and confirmatively residues of 25 β₂-agonists and 21 β-blockers in urine samples. Urine samples were subjected to enzymatic
Michael T Furlong et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 926, 36-41 (2013-04-02)
Clopidogrel is prescribed for the treatment of Acute Coronary Syndrome and recent myocardial infarction, recent stroke, or established peripheral arterial disease. A sensitive and reliable high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay was developed and validated to enable reliable
Hirotaka Kushida et al.
Biomedical chromatography : BMC, 27(12), 1647-1656 (2013-07-03)
Uncaria Hook (UH) alkaloids are involved in the beneficial effects of Yokukansan. However, the pharmacokinetics of UH alkaloids after oral administration of Yokukansan has not yet been sufficiently investigated. Therefore, we developed and validated a sensitive and specific high-performance liquid

Articles

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