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A5963

Sigma-Aldrich

Anti-c-Myc−Alkaline Phosphatase antibody, Mouse monoclonal

clone 9E10, purified from hybridoma cell culture

Synonym(s):

Anti-c-Myc, Monoclonal Anti-c-Myc−Alkaline Phosphatase antibody produced in mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9E10, monoclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: 1:100 using a myc-tagged fusion protein.

isotype

IgG1

UniProt accession no.

P01106

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... MYC(4609)

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General description

Monoclonal Anti-c-myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/c mouse. The c-myc gene encodes for a protein of 62kDa called p62c-myc is associated mainly with cell nuclei.

Immunogen

synthetic peptide of the human p62c-Myc protein.

Application

Anti-c-Myc-Alkaline Phosphatase antibody has been used in
  • enzyme linked immunosorbent assay (ELISA)
  • immunohistochemical labeling
  • immunostaining
  • western blotting

Monoclonal Anti-c-Myc-Alkaline Phosphatase antibody can be used for western blot assays at 1:100, using myc-tagged fusion proteins. The antibody can also be used for immunohistochemical applications.

Biochem/physiol Actions

The c-myc oncogene product(p62c-myc) exerts normal and oncogenic cellular functions. Elevated level of c-myc protein in malignant tissues when compared with normal tissue, but with the unexpected finding of a cytoplasmic accumulation of the protein in these tumors.

Physical form

Solution in 0.05 M Tris buffer, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
Evan G I, et al.
Molecular and Cellular Biology, 5(12), 3610-3616 (1985)
Structural and functional insights into the lipopolysaccharide ABC transporter LptB 2 FG
Dong H, et al.
Nature Communications, 8(1), 222-222 (2017)
Ubiquitin ligase SYVN1/HRD1 facilitates degradation of the SERPINA1 Z variant alpha-1-antitrypsin Z variant via SQSTM1/p62-dependent selective autophagy
Feng,L, et al.
Autophagy, 13(4), 686-702 (2017)
Haohao Dong et al.
Nature communications, 8(1), 222-222 (2017-08-10)
The cell surface of most Gram-negative bacteria contains lipopolysaccharide that is essential for their viability and drug resistance. A 134-kDa protein complex LptB
Xiaodi Tang et al.
Nature communications, 10(1), 4175-4175 (2019-09-15)
Lipopolysaccharides (LPS) of Gram-negative bacteria are critical for the defence against cytotoxic substances and must be transported from the inner membrane (IM) to the outer membrane (OM) through a bridge formed by seven membrane proteins (LptBFGCADE). The IM component LptB2FG powers the
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