Skip to Content
Merck
  • USP17 is required for clathrin mediated endocytosis of epidermal growth factor receptor.

USP17 is required for clathrin mediated endocytosis of epidermal growth factor receptor.

Oncotarget (2014-07-16)
Jakub Jaworski, Michelle de la Vega, Sarah J Fletcher, Cheryl McFarlane, Michelle K Greene, Andrew W Smyth, Sandra Van Schaeybroeck, James A Johnston, Christopher J Scott, Joshua Z Rappoport, James F Burrows
ABSTRACT

Previously we have shown that expression of the deubiquitinating enzyme USP17 is required for cell proliferation and motility. More recently we reported that USP17 deubiquitinates RCE1 isoform 2 and thus regulates the processing of 'CaaX' motif proteins. Here we now show that USP17 expression is induced by epidermal growth factor and that USP17 expression is required for clathrin mediated endocytosis of epidermal growth factor receptor. In addition, we show that USP17 is required for the endocytosis of transferrin, an archetypal substrate for clathrin mediated endocytosis, and that USP17 depletion impedes plasma membrane recruitment of the machinery required for clathrin mediated endocytosis. Thus, our data reveal that USP17 is necessary for epidermal growth factor receptor and transferrin endocytosis via clathrin coated pits, indicate this is mediated via the regulation of the recruitment of the components of the endocytosis machinery and suggest USP17 may play a general role in receptor endocytosis.

MATERIALS
Product Number
Brand
Product Description

Supelco
L-Glutamine, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
L-Glutamine
Supelco
L-Glutamine, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
L-Glutamine, BioUltra, ≥99.5% (NT)
Sigma-Aldrich
DAPI, for nucleic acid staining
Sigma-Aldrich
L-Glutamine, γ-irradiated, BioXtra, suitable for cell culture
Sigma-Aldrich
L-Glutamine, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source
Sigma-Aldrich
L-Glutamine
Sigma-Aldrich
Anti-EGFR (Ab-1) Mouse mAb (528), lyophilized, clone 528, Calbiochem®
SAFC
L-Glutamine