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Sigma-Aldrich

Phenosafranin

Dye content 80 %

Synonym(s):

3,7-Diamino-5-phenylphenazinium chloride

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About This Item

Empirical Formula (Hill Notation):
C18H15ClN4
CAS Number:
81-93-6
Molecular Weight:
322.79
Colour Index Number:
50200
Beilstein:
3642082
EC Number:
201-387-0
MDL number:
MFCD00036335
UNSPSC Code:
12171500
PubChem Substance ID:
24851930
NACRES:
NA.47

form

powder

composition

Dye content, 80%

technique(s)

titration: suitable

mp

>300 °C (lit.)

λmax

519 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Nc1ccc2nc3ccc(N)cc3[n+](-c4ccccc4)c2c1

InChI

1S/C18H14N4.ClH/c19-12-6-8-15-17(10-12)22(14-4-2-1-3-5-14)18-11-13(20)7-9-16(18)21-15;/h1-11H,(H3,19,20);1H

InChI key

SOUHUMACVWVDME-UHFFFAOYSA-N

Application

Phenosafranin has been used for nuclear staining.

Biochem/physiol Actions

Phenosafranin (PSF) is a red dye released from cells with intact plasma membrane. PSF at lower concentration is used to stain mitochondria supravitally.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBM9214
MKBD5349V
MKBD5349
00522PE
13309TG

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Paramita Das et al.
The journal of physical chemistry. B, 111(38), 11169-11176 (2007-09-06)
A steady-state and time-resolved photophysical study of a cationic phenazinium dye, phenosafranin (PSF), has been investigated in well-characterized biomimetic micellar nanocavities formed by anionic surfactants of varying chain lengths, namely, sodium decyl sulfate (S(10)S), sodium dodecyl sulfate (S(12)S), and sodium
Paramita Das et al.
Journal of colloid and interface science, 320(1), 9-14 (2008-01-15)
We introduce a simple and efficient strategy to enhance the efficiency of a quenching-based fluorosensor for metal ions by several orders of magnitude by using commercially available anionic surfactants varying hydrophobic chain length. Anionic surfactants with a proper choice of
Brian D Jones et al.
Water research, 39(18), 4343-4354 (2005-10-26)
An in situ methodology based on covalently bonded redox indicators has been developed for determining when sulfate-reducing conditions exist in environmental samples. Three immobilized redox indicators [thionine (Thi, formal potential at pH 7 (E(0')7) equals 52 mV), cresyl violet (CV
Ishita Saha et al.
Physical chemistry chemical physics : PCCP, 12(39), 12771-12779 (2010-08-24)
The base specificity and energetics of DNA binding of the phenazinium dyes phenosafranine and safranine-O have been studied using various biophysical tools. The guanine-cytosine base specificity of both compounds was established from binding affinity values and competition dialysis results and
Deboleena Sarkar et al.
The journal of physical chemistry. B, 112(30), 9243-9249 (2008-07-10)
Absorption, steady-state fluorescence, steady-state fluorescence anisotropy, and intrinsic and induced circular dichroism (CD) have been exploited to explore the binding of calf thymus DNA (ctDNA) with three cationic phenazinium dyes, viz., phenosafranin (PSF), safranin-T (ST), and safranin-O (SO). The absorption
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