MHA000P2D

Wallerstein Differential Broth

capacity 2 mL, ampoule used in breweries to detect and enumerate bacteria present in small numbers in a mixed flora sample. Cycloheximide inhibits most yeasts and molds.

• Synonym(s): WLD, WL Differential broth
• UNSPSC Code: 41106225
• eCl@ss: 32160801
• NACRES: NA.74

Properties

• Quality Level: 100
• sterility: sterile
• form: liquid
• shelf life: 12 mo.
• feature: ready-to-use
• packaging: pack of 50 tests
• capacity: 2 mL
• filling volume: 2 mL
• color: slightly opalescent green-blue
• input: sample type sport drink; sample type bottled water; sample type cosmetics; sample type beer; sample type beverage(s); sample type beverage(s); sample type soft drink; sample type cider; sample type food(s); sample type wine
• pH: 5.5±0.2 (25.0 °C)
• application(s): environmental; food and beverages; industrial qc (microbial monitoring); microbiology
• compatibility: for use with MF-method; for use with Microfil^®; for use with MicropreSure^®; for use with Milliflex^®
• shipped in: wet ice
• storage temp.: 2-8°C
• suitability: bacteria

Description

General description

WL Differential media is recommended for selective isolation and enumeration of bacteria encountered in breweries and industrial fermentations. The presence of cyclohexamide inhibits the growth of yeasts.{4} Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment and trained personnel are often in short supply. They are available in convenient, single test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.

Application

WL differential media is used in the growth of acetic acid bacteria, Flavobacterium, Proteus and thermophilic bacteria under aerobic and growth of lactic acid bacteria and Pediococcus under anaerobic condition.{5}

Analysis Note

Bacteria appear as small colonies with green blue color. If cycloheximide resistant yeast grow, they are creamy, green-white

QC organisms
A. aceti (ATCC 15973)

Target Microorganisms: Aciduric bacteria (yeast and mold are inhibited)

Legal Information

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany

MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable