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HPLC Analysis of Oligonucleotides on Proteomix® SAX

HPLC Analysis of Oligonucleotides on Proteomix® SAX application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Proteomix® SAX-NP5 Strong Anion Exchange Column

L × I.D. 25 cm × 4.6 mm, 5 μm particle size

CONDITIONS

column

Proteomix SAX-NP5, 25 cm x 4.6 mm I.D., 5 μm particles (Z777229)

mobile phase

A: 20 mM Tris, pH 8.0 B: A + 0.5 M NaCl

gradient

0 - 100% B in 30 minutes

flow rate

0.5 mL/min

pressure

1073 psi (74 bar)

column temp.

25 °C

detector

UV 260 nm

injection

5 μL

sample

(10 mM each oligonucleotide in water)

Description

Legal Information

Proteomix is a registered trademark of Sepax Technologies