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  • Dissociation of neopterin and 7,8-dihydroneopterin from plasma components before HPLC analysis.

Dissociation of neopterin and 7,8-dihydroneopterin from plasma components before HPLC analysis.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2008-02-01)
Elizabeth A Flavall, Elizabeth M Crone, Grant A Moore, Steven P Gieseg
ABSTRACT

Measurement of plasma neopterin by HPLC with fluorescence detection is used clinically as a marker of immune cell activation in the management of a number of disease pathologies. HPLC analysis of neopterin requires the acidic removal of plasma proteins but we have found that 7,8-dihydroneopterin is oxidised to neopterin with varying yield. Using acetonitrile as the precipitant, we have measured substantially higher quantities of both total neopterin (7,8-dihydroneopterin and neopterin) and neopterin from plasma of healthy and septicemia patient's. Total neopterin concentrations were on average 50% and 200% greater in healthy and septicemia subjects, respectively, when measured after acetonitrile precipitation compared to trichloroacetic acid. Our data suggests that some pterin co-precipitates with proteins during acid treatment.

MATERIALS
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Product Description

Sigma-Aldrich
7,8-Dihydroneopterin, ≥97.0% (HPLC)
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