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Merck

An automated system for high-throughput single cell-based breeding.

Scientific reports (2013-02-05)
Nobuo Yoshimoto, Akiko Kida, Xu Jie, Masaya Kurokawa, Masumi Iijima, Tomoaki Niimi, Andrés D Maturana, Itoshi Nikaido, Hiroki R Ueda, Kenji Tatematsu, Katsuyuki Tanizawa, Akihiko Kondo, Ikuo Fujii, Shun'ichi Kuroda
ABSTRACT

When establishing the most appropriate cells from the huge numbers of a cell library for practical use of cells in regenerative medicine and production of various biopharmaceuticals, cell heterogeneity often found in an isogenic cell population limits the refinement of clonal cell culture. Here, we demonstrated high-throughput screening of the most suitable cells in a cell library by an automated undisruptive single-cell analysis and isolation system, followed by expansion of isolated single cells. This system enabled establishment of the most suitable cells, such as embryonic stem cells with the highest expression of the pluripotency marker Rex1 and hybridomas with the highest antibody secretion, which could not be achieved by conventional high-throughput cell screening systems (e.g., a fluorescence-activated cell sorter). This single cell-based breeding system may be a powerful tool to analyze stochastic fluctuations and delineate their molecular mechanisms.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Silicone oil, viscosity 1,000 cSt (25 °C)
Sigma-Aldrich
Silicone oil, viscosity 100,000 cSt (25 °C)
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Silicone oil, viscosity 50 cSt (25 °C)
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Silicone oil, viscosity 60,000 cSt (25 °C)
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Silicone oil, viscosity 10 cSt (25 °C)
Silicone oil, for melting point and boiling point apparatuses
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Silicone oil, viscosity 20 cSt (25 °C)
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Silicone oil, viscosity 10,000 cSt (25 °C)
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Silicone oil, viscosity 350 cSt (25 °C)
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Silicone oil, viscosity 30,000 cSt (25 °C)
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Silicone oil, for oil baths (from −50°C to +200°C)
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Silicone oil, viscosity 100 cSt (25 °C)
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Silicone oil, viscosity 5 cSt (25 °C)
Sigma-Aldrich
Silicone oil, viscosity 500 cSt (25 °C)