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  • Leiomyoma phosphoproteins involved in inhibition of oxidative stress and synthesis of reactive oxygen species.

Leiomyoma phosphoproteins involved in inhibition of oxidative stress and synthesis of reactive oxygen species.

International journal of molecular medicine (2019-10-23)
Blendi Ura, Lorenzo Monasta, Giorgio Arrigoni, Danilo Licastro, Giovanni Di Lorenzo, Federico Romano, Bartolomea Gaita, Federica Scrimin, Giuseppe Ricci
ABSTRACT

Uterine leiomyomas are benign smooth muscle cell tumors originating from the myometrium. The present study focused on leiomyoma and myometrium phosphoproteome enrichment by using immobilized metal affinity chromatography (IMAC). The phosphoproteome was analyzed by two‑dimensional gel electrophoresis coupled with mass spectrometry. Western blotting was used for data validation. The results from IMAC identified 26 proteins significantly differentially phosphorylated in leiomyomas compared with normal myometrium. Three upregulated proteins (peroxiredoxin 2, protein disulfide isomerase family A member 3 and peroxiredoxin 4) were further validated by western blotting. Ingenuity pathway analysis revealed that four phosphoproteins were involved in the inhibition of oxidative stress and synthesis of reactive oxygen species. The present results demonstated for the first time an association between oxidative stress and phosphorylation in leiomyoma development.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-PRDX4, affinity isolated antibody
Sigma-Aldrich
Anti-PRDX2 antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-Goat IgG (whole molecule) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution