I3146
ITS Liquid Media Supplement (100×)
liquid, sterile-filtered, BioReagent, suitable for cell culture
Synonym(s):
Human ITS Liquid Media, Insulin-transferrin-sodium selenite
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About This Item
UNSPSC Code:
12352205
NACRES:
NA.75
Recommended Products
sterility
sterile-filtered
product line
BioReagent
form
liquid
technique(s)
cell culture | mammalian: suitable
single cell analysis: suitable
impurities
endotoxin, tested
shipped in
ambient
storage temp.
2-8°C
Application
ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:
- α mouse liver 12 (AML-12) cells
- rat fetal testis explants
- human dental pulp stem cells (DPSC)
Biochem/physiol Actions
- Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.
- Transferrin: an iron-transport protein.
- Selenium: an essential trace element normally provided by serum.
Components
Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.
Other Notes
ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.
Preparation Note
Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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