E9034

Phosphodiesterase 5A1 human

recombinant, expressed in Sf9 cells

• Synonym(s): 3′,5′-cyclic-GMP phosphodiesterase, PDE 5A1
• Enzyme Commission number: 3.1.4.25 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in Sf9 cells
• Quality Level: 200
• form: solution
• specific activity: ≥2,000 unit/μg protein
• mol wt: 126 kDa
• UniProt accession no.: O76074
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PDE5A(8654)

Description

General description

Research area: Cell SignallingPDE5is located in various tissues such as the kidney, brain, lungs, cardiac myocytes,vascular smooth muscle cells, penile corpus cavernosum, platelets, andgastrointestinal tissues.

Biochem/physiol Actions

PDE5 regulates vascular smooth muscle contraction and is involved in NO-cGMP signaling in platelets to control aggregation. PDE5 may also be involved in the regulation of cGMP signaling in the brain and may modulate pressure-induced cardiac hypertrophy and fibrosis . Phosphodiesterase (PDE)is an enzyme that is used to break phosphodiester bonds. Phosphodiesterase 5 (PDE5), amolecular target for the treatment of erectile dysfunction and pulmonaryhypertension. PDE5 is used to find novel PDE5 inhibitors. PDE5 specifically targets cyclicguanosine monophosphate (cGMP) generated by the activation of nitric oxide (NO). PDE5 regulates cGMP signalingin the cardiovascular and other tissues. At the genetic level PDE5 isexpressed as various isoforms and undergoes phosphorylation as a post-translationalmodification that results in its activation. PDE5 is degraded through theubiquitin pathway due to its nitrosylation.

Physical properties

N-terminal GST-tagged 126 kDa full-length protein

Unit Definition

One unit will convert 1.0 picomole of 3′,5′-cGMP to 5′-GMP per minute at pH 7.4 at 37 °C.

Physical form

Supplied as a solution in 20% glycerol containing 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% TWEEN^® 20 and 3 mM DTT

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable