MABD24
Anti-NANOG Antibody, clone 7F7.1
clone 7F7.1, from mouse
Synonym(s):
Homeobox protein NANOG, Homeobox transcription factor Nanog, hNanog
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
7F7.1, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NANOG(79923)
General description
NANOG (Homeobox protein NANOG) is a member of the Nanog homeobox family of DNA-binding proteins. It is expressed in embryonic stem cells and confers pluripotency on these cells. Once embryonic stem cells become differentiated, NANOG expression is suppressed. NANOG is involved in the Hedgehog/Gli1 signaling pathway which has been implicated in the development and growth of various types of tumors.
Immunogen
GST-tagged recombinant protein corresponding to human NANOG.
Application
Anti-NANOG Antibody, clone 7F7.1 is an antibody against NANOG for use in Western Blotting, ICC.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NANOG in H9 human embryonic stem cells.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Evaluated by Western Blot in H9 human embryonic stem cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected NANOG on 10 µg of H9 human embryonic stem cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected NANOG on 10 µg of H9 human embryonic stem cell lysate.
Target description
~39 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
H9 human embryonic stem cell lysate
H9 human embryonic stem cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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