Mammalian Cell Lines
We are partnered with the European Collection of Authenticated Cell Cultures (ECACC) repository, part of Public Health England, to offer quality-controlled cell lines and nucleic acids to laboratories around the world. Browse our portfolio of authenticated, validated, mycoplasma-free cell lines for biomedical research, protein production, hybridoma fusion, virus propagation, and more—including hundreds of cancer cell lines, and iPS cells from the European Bank of induced Pluripotent Stem Cells (EBiSC). Cell line product pages also include ordering information for DNA, RNA and cDNA derived from corresponding cells, which allow researchers to screen for genes or gene expression patterns and identify the cell lines most suitable for their research.
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Cell Lines
Our comprehensive offering of cells available with easy ordering and fast delivery also includes unique primate cell lines collected in accordance with guidelines established by the Convention on International Trade in Endangered Species (CITES), as well as the Human Leukocyte Antigen (HLA) typed collection, and hybridoma cell lines that produce monoclonal antibody against specific epitopes.
Our encyclopedic collection of cells includes lines from the following tissue types and species:
Search cell lines by cancer type:
To ensure reliable propagation and reproducible results, we recommend using authenticated cell lines from a reputable source. The Sigma-Aldrich® brand guarantees unsurpassed quality control, so the cell lines we offer are pathogen-free and identity-certified. Browse our collection of over 600 cancer cell lines.
Other Mammalian Cell Line Collections
Our extensive collection of cell lines goes beyond tissue-specific human and cancer cell lines.
- Non-human primate cells in cooperation with CITES: cells collected in cooperation with The Convention on the International Trade in Endangered Species of Wild Fauna and Flora
- HLA Collection: Human Leukocyte Antigen (HLA)-Typed Collection maintained by the European Collection of Cell Cultures (ECACC)
- Hybridoma collection: cell lines optimized for the production of immunoglobulin and used in applications such as monoclonal antibody production
- EBiSC iPS cell lines: inducible pluripotent stem cells from the European Bank
- USCF Lung Epithelial cell lines: these cells are widely used to model barrier function of the airway epithelium and to study respiratory ion transport as well as the function of CFTR. These unique cell lines have also been used to study viral attachment and replication in SARS-CoV-2 infection.
Applications for Mammalian Cell Lines
Cell lines are used not only to model biological systems and disease, but for practical biotechnology uses in the production of proteins, viruses, and more. Explore cells used in these applications:
- Hybridoma fusion partners
- Recombinant protein production
- Retroviral packaging line
- Virus cultivation
Related Product Resources
- Protocol: Thawing of Frozen Cell Lines
Cell culture protocol for proper thawing of cryopreserved cell lines.
- Protocol: Cryopreservation of Cell Lines
Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO.
- Handbook: Fundamental Techniques in Cell Culture Laboratory Handbook – 4th Edition
The 4th Edition Laboratory handbook created from the partnership of the European Collection of Authenticated Cell Cultures (ECACC) and MilliporeSigma.
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