Skip to Content
MilliporeSigma
All Photos(3)

Key Documents

View All Documentation

N8016

Sigma-Aldrich

2-Nitrophenyl β-D-glucopyranoside

≥99% (TLC), powder

Synonym(s):

2-Nitrophenyl-beta-D-glucopyranoside, o-NPG

Sign Into View Organizational & Contract Pricing

Select a Size

250 MG
$193.00
1 G
$586.00

$193.00

In StockDetails

All Photos(3)

Select a Size

Change View
250 MG
$193.00
1 G
$586.00

About This Item

Empirical Formula (Hill Notation):
C12H15NO8
CAS Number:
2816-24-2
Molecular Weight:
301.25
Beilstein/REAXYS Number:
92206
EC Number:
220-568-5
MDL number:
MFCD00065048
UNSPSC Code:
12352204
PubChem Substance ID:
24897848
NACRES:
NA.28

$193.00

In StockDetails

Product Name

2-Nitrophenyl β-D-glucopyranoside, powder

assay

≥99% (TLC)

Quality Level

300

form

powder

solubility

water: 50 mg/mL

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9-,10+,11-,12-/m1/s1

InChI key

KUWPCJHYPSUOFW-RMPHRYRLSA-N

Looking for similar products? Visit Product Comparison Guide

Application

2-Nitrophenyl β-D-glucopyranoside has been used as a substrate for glucosidase in protein assay[1] and in a colorimetric assay to determine protein stability.[2]

Biochem/physiol Actions

2-Nitrophenyl β-D-glucopyranoside serves as an artificial substrate for glucosidase.[1]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000394975
0000389002
0000376994
0000335141
0000335142

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Neil J Nosworthy et al.
Enzyme and microbial technology, 54, 20-24 (2013-11-26)
We have achieved plasma immersion ion implantation (PIII) treatment of beads and powders using a specially designed plasma treatment system. This simple one-step production of functionalized beads provides an attractive alternative to current commercial functional beads, for which proteins must
J H Lebbink et al.
Biochemistry, 39(13), 3656-3665 (2000-03-29)
The beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus (CelB) is the most thermostable and thermoactive family 1 glycosylhydrolase described to date. To obtain more insight in the molecular determinants of adaptations to high temperatures and study the possibility of optimizing
Antonio Trincone et al.
Chembiochem : a European journal of chemical biology, 6(8), 1431-1437 (2005-07-13)
Transglycosylation reactions (autocondensation of the substrate or transfer of the glycon donor moiety to different acceptors) with the hyperthermophilic glycosynthase from Sulfolobus solfataricus acting in dilute sodium formate buffer at pH 4.0 are reported; the use of 4-nitrophenyl beta-glucopyranoside as
L M Hernandez et al.
FEBS letters, 161(2), 190-194 (1983-09-19)
Accumulation and secretion of beta-glucanases have been studied in vivo by using a thermosensitive secretory mutant of Saccharomyces cerevisiae blocked at the endoplasmic reticulum level (sec 18-1). When incubated at the restrictive temperature no accumulation of active glucanases was observed.
Toshihiro Ohta et al.
Mutagenesis, 21(4), 255-260 (2006-06-17)
Thermus thermophilus is an extremely thermophilic eubacterium that grows optimally at 70-75 degrees C. Because the frequency of DNA damage, such as deamination, depurination and single-strand breaks, increases as the temperature rises, the regulation of expression as well as the
View All Related Papers

Articles

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Questions

  1. hello, is this also a chromogenic substrate?

    1 answer

    1. Yes, this compound is considered to be a chromogenic beta-glucosidase substrate. Application testing is not performed on this product. Please see the links below to review publications that may be helpful.

      Characterization of a heat resistant ß-glucosidase as a new reporter in cells and mice
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2909947/

      Ion implantation treatment of beads for covalent binding of molecules: Application to bioethanol production using thermophilic beta-glucosidase
      https://www.sciencedirect.com/science/article/pii/S0141022913002019?via%3Dihub

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service