M5520
Myokinase from chicken muscle
essentially salt-free, lyophilized powder, 1,500-3,000 units/mg protein (biuret)
Synonym(s):
ATP: AMP phosphotransferase, Adenylate Kinase
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About This Item
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form
essentially salt-free, lyophilized powder
specific activity
1,500-3,000 units/mg protein (biuret)
foreign activity
3-phosphoglyceric phosphokinase ≤0.1%
lactic dehydrogenase ≤0.02%
pyruvate kinase ≤0.05%
storage temp.
−20°C
General description
Mutational analysis of the amino acid proline 17 of myokinase from chicken muscle is critical for structural stability, substrate binding and enzyme activity. [1]
Unit Definition
One unit will convert 2.0 μmoles of ADP to ATP+AMP per min at pH 7.6 at 37°C.
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Koji Kasai et al.
Nucleic acids research, 32(19), 5732-5741 (2004-10-28)
Chloroplasts possess bacterial-type systems for transcription and translation. On the basis of the identification of a Chlamydomonas reinhardtii gene encoding a RelA-SpoT homolog (RSH) that catalyzes the synthesis of guanosine tetra- or pentaphosphate [(p)ppGpp], we have previously suggested the operation
E Aboulmagd et al.
Applied and environmental microbiology, 67(5), 2176-2182 (2001-04-25)
Synechocystis sp. strain PCC6308 cyanophycin synthetase was purified 72-fold in three steps by anion exchange chromatography on Q Sepharose, affinity chromatography on the triazine dye matrix Procion Blue HE-RD Sepharose, and gel filtration on Superdex 200 HR from recombinant cells
Yuki Goto et al.
Nature protocols, 6(6), 779-790 (2011-06-04)
Genetic code reprogramming is a method for the reassignment of arbitrary codons from proteinogenic amino acids to nonproteinogenic ones; thus, specific sequences of nonstandard peptides can be ribosomally expressed according to their mRNA templates. Here we describe a protocol that
María J López Barragán et al.
Journal of bacteriology, 186(17), 5762-5774 (2004-08-20)
We report here that the bzd genes for anaerobic benzoate degradation in Azoarcus sp. strain CIB are organized as two transcriptional units, i.e., a benzoate-inducible catabolic operon, bzdNOPQMSTUVWXYZA, and a gene, bzdR, encoding a putative transcriptional regulator. The last gene
A Martonosi et al.
The Journal of biological chemistry, 252(1), 318-332 (1977-01-10)
The development of sarcoplasmic reticulum membranes was studied in vivo and in tissue culture in chicken pectoralis muscle cells. The concentration of the calcium- and magnesium-activated ATPase measured by selective labeling of the enzyme with [32P]ATP in whole muscle homogenates
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