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E1388

β-Endothelial Cell Growth Factor human

Name: β-Endothelial Cell Growth Factor human
Brand: SIGMA

β-ECGF, recombinant, expressed in E. coli, suitable for cell culture

Synonym(s):

ECGS, β-ECGF

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About This Item

CAS Number:

105843-51-4

MDL number:

MFCD00131012

UNSPSC Code:

12352202

NACRES:

NA.77

biological source

human

recombinant

expressed in E. coli

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

0.03-0.3 ng/mL ED₅₀/EC₅₀

quality

endotoxin tested

mol wt

predicted mol wt ~17 kDa

packaging

pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)

technique(s)

cell culture | mammalian: suitable

impurities

≤1.0 EU/μg protein Endotoxin level (LAL test)

UniProt accession no.

P49765

storage temp.

−20°C

Gene Information

human ... VEGFB(7423)

Related Categories

Cell Culture & Analysis

Cell Culture Supplements & Reagents

Growth Factors and Cytokines

Molecular Biology & Functional Genomics

General description

The β-endothelial cell growth factor (b-ECGF) gene encodes an angiogenic factor, and is mapped to human chromosome 11q13.1. Recombinant, human b-endothelial cell growth factor (b-ECGF) is a 14 amino acid N-terminal extension of acidic fibroblast growth factor. It is expressed in Escherichia coli. The 154 amino acid recombinant protein, generated after removal of the initiation methionine, has a predicted molecular mass of about 17 kDa.

application

β-Endothelial Cell Growth Factor human has been used:

in cell death assay
in endothelial cell culture
in human umbilical vascular endothelial cell (HUVEC) culture

Biochem/physiol Actions

β-Endothelial cell growth factor (β-ECGF) is a potent endothelial cell mitogen and its mitogenic activity is potentiated by heparin. β-ECGF stimulates the growth of quiescent HUVEC cells in culture. β-ECGF is associated with lipid and glucose metabolism. This growth factor induces neovascularization in disease conditions. Upregulation of β-ECGF is observed in diabetic retinopathy, and polycystic ovary syndrome. It is also associated with tumor proliferation and invasion. β-ECGF increases fatty acid transport proteins expression to improve endothelial mediated lipid transpor.

Other Notes

14 amino acid, N-terminal extended form of human FGF-acidic.

Physical form

Lyophilized from a 0.2 μm filtered solution in 10 mM MOPS, 50 mM ammonium sulfate, 0.2 mM DTT, and 0.2 mM EDTA, pH 7.2, containing 50 μg bovine serum albumin per 1 μg cytokine.

Analysis Note

The proliferative activity is tested in culture by using quiescent NR6R-3T3 fibroblasts.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c