Basic Cell Culture Protocols 3rd ed.

This third edition offers today′s most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. This book should be in every cell culture laboratory and be of use to all who use cell cultures in research

1 Culture of Primary Adherent Cells and a Continuously Growing Non-Adherent Cell Line
2 Detection of Mycoplasma Contaminations
3 Eradication of Mycoplasma Contaminations
4 Authentication of Scientific Human Cell Lines: Easy to Use DNA Fingerprinting
5 Cytogenetic Analysis of Cell Lines
6 Human and Mouse Hematopoietic Colony Forming Cell Assays
7 Isolation and Culture of Murine Macrophages
8 Isolation and Culture of Human Macrophages
9 The Development of T Lymphocytes in Mouse Fetal Thymus Organ Culture
10 In Vitro Generation of Lymphocytes from Embryonic Stem Cells
11 Hematopoietic Development of Human Embryonic Stem Cells in Culture
12 Generation of Murine Stromal Cell Lines: Models for the Microenvironment of the Embryonic Mouse Aorta-Gonads-Mesonephros (AGM) region
13 Culture of Human and Mouse Mesenchymal Cells
14 Isolation, Purification, and Cultivation of Rat and Human Keratinocytes
15 Isolation and Culture of Primary Human Hepatocytes
16 Primary Kidney Proximal Tubule Cells
17 Enzymatic Dissociation and Culture of Normal Human Mammary Tissue to Detect Progenitor Activity
18 Generation and Differentiation of Neurospheres from Murine Embryonic Day 14 Central Nervous System Tissue
19 Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells
20 Adult Ventricular Cardiomyocytes: Isolation and Culture
21 Isolation and Culture of Primary Endothelial Cells
22 Studying Leukocyte Rolling and Adhesion In Vitro Under Flow Conditions
23 Isolation and Characterization of SP Cells
24 Scalable Production of Embryonic Stem Cell-Derived Cells