Culture of Animal Cells, 4th ed.

This edition updates and expands the scope of its predecessor by providing proven methods for the culture and experimental manipulation of animal cells, laboratory procedures, reagent preparation, and equipment and supply information. Particular attention has been focused on issues of safety, biohazards, and automation in the cell culture laboratory.

Biology of the Cultured Cell.  
Design and Layout of the Laboratory.  
Equipping the Laboratory.  
Aseptic Technique.  
Laboratory Safety and Biohazards.  
The Culture Environment: Substrate, Gas Phase, Medium and Temperature.  
Preparation and Sterilization.  
Disaggregation of the Tissue and Primary Culture.  
Maintenance of the Culture:
Cell Lines.  
Cloning and Selection of Specific Cell Types.  
Physical Methods of Cell Separation.  
Characterization.  
Induction of Differentiation.  
The Transformed Phenotype.  
Contamination.  
Instability, Variation, and Preservation.  
Quantitation and Experimental Design.  
Measurement of Viability and Cytotoxicity.  
Culture of Specific Cell Types.  
Culture of Tumor Tissue.  
Three-Dimensional Culture Systems.  
Specialized Techniques.  
Reagent Appendix.  
Glossary.  
References.
Indexes.