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V4383

Sigma-Aldrich

Vimentin from bovine lens

lyophilized powder

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

bovine eye (lens)

Quality Level

assay

≥98% (SDS-GE)

form

lyophilized powder

mol wt

~57 kDa

UniProt accession no.

storage temp.

−20°C

Gene Information

bovine ... VIM(280955)

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Other Notes

A component of mesenchymal cell intermediate filaments.

Reconstitution

When reconstituted with distilled water at 1 mg vimentin per mL, the solution will contain 10 mM sodium phosphate, pH 7.5, 2 mM dithiothreitol, 1 mM EDTA, 6 M urea, and 10 mM methylammonium chloride.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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H Bloemendal et al.
FEBS letters, 180(2), 181-184 (1985-01-28)
A novel, simple and relatively rapid method is described for the isolation of the intermediate-sized filament protein vimentin from eye lens tissue. Chromatofocusing is applied as the sole purification step. The apparent isoelectric point of the protein in 6 M
Youngjoo Kwon et al.
PloS one, 6(4), e18872-e18872 (2011-04-29)
Epithelial ovarian cancer is an aggressive and deadly disease and understanding its invasion mechanisms is critical for its treatment. We sought to study the penetration/invasion of ovarian tumor cells into extracellular matrices (ECMs) using a fibroblast-derived three-dimensional (3D) culture model

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