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T7408

Sigma-Aldrich

Triethylammonium bicarbonate buffer

1.0 M, pH 8.5±0.1

Synonym(s):

Triethylammonium hydrogen carbonate buffer

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About This Item

CAS Number:
15715-58-9
Beilstein/REAXYS Number:
3624751
MDL number:
MFCD00466938
UNSPSC Code:
12161700
PubChem Substance ID:
24900453
NACRES:
NA.25

form

liquid

storage condition

(Tightly closed)

concentration

1.0 M

color

colorless

pH

8.5±0.1

density

1.02 g/mL at 25 °C (lit.)

suitability

suitable for LC-MS
suitable for mass spectrometry

application(s)

diagnostic assay manufacturing
microbiology
sample preparation

storage temp.

2-8°C

SMILES string

OC(O)=O.CCN(CC)CC

InChI

1S/C6H15N.CH2O3/c1-4-7(5-2)6-3;2-1(3)4/h4-6H2,1-3H3;(H2,2,3,4)

InChI key

AFQIYTIJXGTIEY-UHFFFAOYSA-N

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Application

Triethylammonium bicarbonate buffer is used in ion-exchange chromatography and electrophoresis.
It has also been used:
  • in trypsin digestion
  • as a component of lysis buffer
  • to adjust the pH of buffer for protein quantification

Buffer for use in ion-exchange chromatography and electrophoresis.

Biochem/physiol Actions

Triethylammonium bicarbonate buffer is useful in oligonucleotides purification for electrospray mass spectrometry analysis. It is also utilized in trypsin digestion buffers.

Other Notes

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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Certificates of Analysis (COA)

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Evaluation of the Effect of Trypsin Digestion Buffers on Artificial Deamidation
Hao P, et al.
Journal of Proteome Research, 14(2), 1308-1314 (2015)
Stefani N Thomas et al.
iScience, 23(6), 101079-101079 (2020-06-14)
The National Cancer Institute (NCI) Clinical Proteomic Tumor Analysis Consortium (CPTAC) established a harmonized method for large-scale clinical proteomic studies. SWATH-MS, an instance of data-independent acquisition (DIA) proteomic methods, is an alternate proteomic approach. In this study, we used SWATH-MS
Differential protein expression analysis of degenerative aortic stenosis by iTRAQ labeling.
Alonso-Orgaz S, et al.
Methods in Molecular Biology, 109-117 (2013)
Identification of Rosmarinic Acid-Adducted Sites in Meat Proteins in a Gel Model under Oxidative Stress by Triple TOF MS/MS.
Tang CB, et al.
Journal of Agricultural and Food Chemistry, 64(33), 6466-6476 (2016)
C G Huber et al.
Journal of mass spectrometry : JMS, 35(7), 870-877 (2000-08-10)
The applicability of ion-pair reversed-phase high-performance liquid chromatography/electrospray ionization mass spectrometry (IP-RP-HPLC/ESI-MS) and direct infusion/ESI-MS to the characterization of nucleic acid mixtures was evaluated by the analysis of the reaction products obtained from solid-phase synthesis of a 39-mer oligonucleotide. IP-RP-HPLC/ESI-MS
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