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T5425

Sigma-Aldrich

Transforming Growth Factor-β3 human

TGF-β3, recombinant, expressed in baculovirus infected Sf21 cells, powder, suitable for cell culture

Synonym(s):

hTGF-β3, TGF-β3

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About This Item

MDL number:
MFCD00213054
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

Quality Level

100

recombinant

expressed in baculovirus infected Sf21 cells

assay

≥97% (SDS-PAGE)

form

powder

potency

0.01-0.09 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

predicted mol wt ~25 kDa

packaging

pkg of 2 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

<0.01 EU/μg

UniProt accession no.

P10600

storage temp.

−20°C

Gene Information

human ... TGFB3(7043)

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General description

The TGFβ3 (transforming Growth Factor-β3) gene is mapped to human chromosome 14q24.3.

Application

Transforming Growth Factor-β3 human has been used to study the role of TGF-β3 in healing tendon-to-bone insertion and in chondrogenic differentiation.

Biochem/physiol Actions

TGF-β (transforming growth factor-β3) isoforms are significantly involved in inducing inflammatory responses. It is associated with vascular diseases, wound healing and ameliorating effect in venous leg ulcer. TGF-β3 has an antagonistic effect on TGF-β1 and reduces the degree of collagen type I deposition and fibroblast differentiation. As a result TGF-β3 might lead to ulcer chronicity due to hindered myofibroblasts formation. TGF-β is a key player in tumor formation and progression. Increased TGF-β3 expression is observed in cancer and adipose tissue fibrosis. TGF-β3 is crucial in tissue regeneration and scarless tissue repair.
TGF-β3 is less prevalent in natural expression than either TGF-β1 or TGF-β2, but it is the most abundant mRNA expressed in chick embryos. It is also expressed in human umbilical cord, in a variety of mesenchymal cells of human and rodent origin, and in several human carcinoma cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in 35% acetonitrile and 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg of cytokine.

Analysis Note

The biological activity is measured by its ability to inhibit the IL-4-dependent proliferation of mouse HT-2 cells.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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Adipose tissue fibrosis in human cancer cachexia: the role of TGFβ pathway
Alves MJ, et al.
BMC Cancer, 17: 190 (2017)
Differential expression of TGF isoforms in breast cancer highlights different roles during breast cancer progression.
Hachim MY, et al.
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 40(1) (2018)
Chondrogenic Differentiation of Menstrual Blood-Derived Stem Cells on Nanofibrous Scaffolds
Kazemnejad S, et al.
Methods in Molecular Biology, 149-169 (2013)
The role of transforming growth factor β isoforms in tendon-to-bone healing.
Kim HM, et al.
Connective tissue research, ;52(2), 87-98 (2011)
Tsang, M., et al.
Proc. 7th Intl. Lymphokine Workshop, 9, 607-607 (1990)
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