S2937
Anti-Sos1 antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
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About This Item
MDL number:
UNSPSC Code:
12352203
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 170 kDa
species reactivity
human, canine, rat
technique(s)
indirect immunofluorescence: 1:500 using using cultured canine MDCK cells.
microarray: suitable
western blot: 1:4,000 using whole extracts of A431 (human epidermoid carcinoma) and PC 12 (rat pheochromocytoma) cell lines.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SOS1(6654)
mouse ... Sos1(20662)
General description
Son of Sevenless (SOS) was first discovered in Drosophila, responsible for normal eye development. Two homologs in mouse and humans have been described, Sos-1 and Sos-2; mouse Sos-1 and Sos-2 are 70% identical. Mammalian Sos proteins are present in the inner cell membrane. Sos-1 is a dual guanine nucleotide exchange factor that activates Rac1 and Ras in response to growth factors. Sos-1 can associate also with the GRAP adaptor protein and it is also capable of forming complexes that exhibit Rac-specific guanine nucleotide exchange factor activity. Along with receptor tyrosine kinases, Sos-1 plays an important role in cellular migration and proliferation. In plants, Sos-1 is important to maintain salt concentration across the cell membranes.
Anti-Sos-1 specifically recognizes Sos-1 by immunoblotting (170 kDa). An additional band of lower molecular weight may be detected in some cell line extracts.
Anti-Sos-1 specifically recognizes Sos-1 by immunoblotting (170 kDa). An additional band of lower molecular weight may be detected in some cell line extracts.
Immunogen
synthetic peptide corresponding to the C-terminus region of SOS-1 of human origin (amino acids 1315-1333). This sequence is identical in mouse.
Application
Detection of Sos-1 by immunoblotting is possible by using a minimum antibody working dilution of 1:4,000 in whole extracts of A431 (human epidermoid carcinoma) and PC 12 (rat pheochromocytoma) cell lines. For indirect immunofluorescent staining a minimum working dilution of 1:500 may be used in cultured canine MDCK cells. The antibody is suitable for protein microarray.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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A S Nimnual et al.
Science (New York, N.Y.), 279(5350), 560-563 (1998-02-07)
The Son of Sevenless (Sos) proteins control receptor-mediated activation of Ras by catalyzing the exchange of guanosine diphosphate for guanosine triphosphate on Ras. The NH2-terminal region of Sos contains a Dbl homology (DH) domain in tandem with a pleckstrin homology
Paulo Silva et al.
Plant signaling & behavior, 4(8), 718-726 (2009-10-13)
Over the last decades several efforts have been carried out to determine the mechanisms of salt homeostasis in plants and, more recently, to identify genes implicated in salt tolerance, with some plants being successfully genetically engineered to improve resistance to
G Scita et al.
Nature, 401(6750), 290-293 (1999-09-28)
The small guanine nucleotide (GTP)-binding protein Rac regulates mitogen-induced cytoskeletal changes and c-Jun amino-terminal kinase (JNK), and its activity is required for Ras-mediated cell transformation. Epistatic analysis placed Rac as a key downstream target in Ras signalling; however, the biochemical
Hyun Sub Hwang et al.
The Journal of cell biology, 195(3), 377-386 (2011-11-02)
Son of sevenless 1 (SOS1) is a dual guanine nucleotide exchange factor (GEF) that activates the guanosine triphosphatases Rac1 and Ras, which mediate signaling initiated by peptide growth factors. In this paper, we show that CIIA is a new binding
Stéphane Pierre et al.
Biochemical pharmacology, 82(9), 1049-1056 (2011-07-27)
Son of Sevenless (SOS) was discovered in Drosophila melanogaster. Essential for normal eye development in Drosophila, SOS has two human homologues, SOS1 and SOS2. The SOS1 gene encodes the Son of Sevenless 1 protein, a Ras and Rac guanine nucleotide
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