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S2907

Sigma-Aldrich

Salicylate Hydroxylase from Pseudomonas sp.

lyophilized powder, 0.5-3.0 units/mg protein

Synonym(s):

Salicylate 1-monooxygenase, Salicylate, NADH:oxygen oxidoreductase (1-hydroxylating, decarboxylating)

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204

form

lyophilized powder

specific activity

0.5-3.0 units/mg protein

composition

Protein, 35-65% biuret

storage temp.

−20°C

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Unit Definition

One unit will convert 1.0 μmole of salicylate and β-NADH to catechol and β-NAD per min at pH 7.6 at 30 °C.

Physical form

Lyophilized powder containing Tris phosphate and FAD

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Thimmaraju Rudrappa et al.
Planta, 226(2), 283-297 (2007-06-08)
Bacillus subtilis, a plant growth promoting rhizobacteria (PGPR), induces growth response and protection against pathogenic organisms through colonization and biofilm formation on the Arabidopsis thaliana root surface. In the current investigation, we utilized various Arabidopsis defense pathway mutants in a
Yves Jouanneau et al.
Applied and environmental microbiology, 73(23), 7515-7521 (2007-10-02)
In the bacterial degradation of polycyclic aromatic hydrocarbons (PAHs), salicylate hydroxylases catalyze essential reactions at the junction between the so-called upper and lower catabolic pathways. Unlike the salicylate 1-hydroxylase from pseudomonads, which is a well-characterized flavoprotein, the enzyme found in
Josefa M Alamillo et al.
The Plant journal : for cell and molecular biology, 48(2), 217-227 (2006-10-05)
Plum pox virus (PPV) is able to replicate in inoculated leaves of Nicotiana tabacum, but is defective in systemic movement in this host. However, PPV produces a systemic infection in transgenic tobacco expressing the silencing suppressor P1/HC-Pro from tobacco etch
Beatriz Cámara et al.
Journal of bacteriology, 189(5), 1664-1674 (2006-12-19)
Pseudomonas sp. strain MT1 has recently been reported to degrade 4- and 5-chlorosalicylate by a pathway assumed to consist of a patchwork of reactions comprising enzymes of the 3-oxoadipate pathway. Genes encoding the initial steps in the degradation of salicylate
Shunji Ueno et al.
Free radical research, 41(11), 1246-1252 (2007-10-02)
Hydroxyl radical (*OH) generation in the kidney of mice treated with ferric nitrilotriacetate (Fe-NTA) or potassium bromate (KBrO3) in vivo was estimated by the salicylate hydroxylation method, using the optimal experimental conditions we recently reported. Induction of DNA lesions and

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