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S2562

Sigma-Aldrich

Anti-Stem Cell Factor Receptor (SCF R) antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym(s):

Anti-CD117

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About This Item

MDL number:
UNSPSC Code:
51111800
NACRES:
NA.41

biological source

goat

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

human

technique(s)

indirect ELISA: 0.5-1.0 μg/mL
neutralization: suitable
western blot: 0.1-0.2 μg/mL

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... KIT(3815)

General description

Mast cells play an important role in innate immunity or the response of the body to invading pathogens. The functions of mast cells are regulated by a number of surface receptors; Stem cell factor receptor (SCF R; CD117) being the most critical. The signalling triggered by SCF R results in mast cell activation in response to antigens. The response involves tyrosine phosphorylation by specific tyrosine kinases that drive the downstream signalling process. The secondary pathways activated by SCF R include Akt, mTOR, MAPK and c-Jun. In addition to development of mast cells, SCF R also regulates their subsequent maturation and homing to the residence sites. Constitutive activation by SCF R is implicated in myeloproliferative discorders, systemic mastocytosis and gastrointestinal stomal cell tumors
Anti-human stem cell factor receptor recognizes recombinant human SCF receptors.

Immunogen

recombinant human stem cell factor receptor (SCF R) extracellular domain expressed in Sf 21 cells.

Application

Anti- stem cell factor receptor antibody may be used to neutralize human SCF R at neutralization dose (ND50) of 0.3-1.0 μg/ml. For ELISA, the recommended antibody concentration is 0.5-1.0 μg/ml. The antibody is suitable for immunoblotting at a working concentration of 0.1-0.2 μg/ml.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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M Tsai et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(14), 6382-6386 (1991-07-15)
We investigated the effects of a newly recognized multifunctional growth factor, the c-kit ligand stem cell factor (SCF), on mouse mast cell proliferation and phenotype. Recombinant rat SCF164 (rrSCF164) induced the development of large numbers of dermal mast cells in
Juan Rivera et al.
The Journal of allergy and clinical immunology, 117(6), 1214-1225 (2006-06-06)
The mast cell is a central player in allergy and asthma. Activation of these cells induces the release of preformed inflammatory mediators localized in specialized granules and the de novo synthesis and secretion of cytokines, chemokines, and eicosanoids. The balance
Alasdair M Gilfillan et al.
Immunological reviews, 228(1), 149-169 (2009-03-18)
Mast cell mediator release represents a pivotal event in the initiation of inflammatory reactions associated with allergic disorders. These responses follow antigen-mediated aggregation of immunoglobulin E (IgE)-occupied high-affinity receptors for IgE (Fc epsilon RI) on the mast cell surface, a
Y Kitamura et al.
International archives of allergy and immunology, 107(1-3), 54-56 (1995-05-01)
Signaling through the c-kit receptor tyrosine kinase (Kit) is essential for development and survival of mast cells but not of basophils. Moreover, we recently found an activation mutation of Kit in several tumor mast cell lines.
Dean D Metcalfe
Blood, 112(4), 946-956 (2008-08-08)
Mast cells have been recognized for well over 100 years. With time, human mast cells have been documented to originate from CD34+ cells, and have been implicated in host responses in both innate and acquired immunity. In clinical immunology, they

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