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S1686

Sigma-Aldrich

Monoclonal Anti-v-Src antibody produced in mouse

clone 327, purified immunoglobulin, lyophilized powder

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.44
conjugate:
unconjugated
application:
IP
WB
clone:
327, monoclonal
species reactivity:
human, rat, mouse
citations:
6
technique(s):
immunoprecipitation (IP): suitable (suitable; may appear as doublet due to phosphorylation)
western blot: 2-5 μg/mL using COLO201 cell extract

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

327, monoclonal

form

lyophilized powder

mol wt

antigen ~56 kDa

species reactivity

human, rat, mouse

technique(s)

immunoprecipitation (IP): suitable (suitable; may appear as doublet due to phosphorylation)
western blot: 2-5 μg/mL using COLO201 cell extract

isotype

IgG1κ

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... SRC(6714)
mouse ... Src(20779)
rat ... Src(83805)

General description

Src family kinases are a group of structurally related non-receptor protein tyrosine kinases that can lead to a variety of cellular responses including differentiation and proliferation. All the members have an amino terminal that has the kinase function and the C-terminal that regulates the kinase function. The amino terminal has Src homology 4 domain (SH4), containing a myristylation signal sequence essential for membrane localization; conserved SH3 and SH2 domains, which mediate protein-protein interactions, separated from the SH4 by a unique region; a catalytic kinase domain. The major difference between v-Src and c-Src is the structure of the C terminal. Src is involved in the regulation of many cellular functions such as migration, adhesion, gene transcription, cell cycle, apoptosis and differentiation. Few signalling pathways through which Src regulates these processes are PI3K, STAT3, FAK and paxillin. Src overexpression is observed in human cancers.
Monoclonal Anti-v-Src reacts with active Src kinase from human, mouse, and rat detecting a band of approximately 56 kDa.

Immunogen

Src protein (p60src).

Application

The recommended anti-v-Src antibody concentration is 2-5 μg/mL for immunoblotting using a COLO201 cell extract and chemiluminescence detection. The antibody is suitable for immunoprecipitation.

Physical form

Lyophilized with 100 μg bovine serum albumin.

Analysis Note

p60src may appear as a doublet due to phosphorylation.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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P T Ram et al.
Oncogene, 20(13), 1601-1606 (2001-04-21)
Extracellular signals when routed through signaling pathways that use heterotrimeric G proteins can engage multiple signaling pathways leading to diverse biological consequences. One locus at which signal sorting occurs is at the level of G proteins. G protein alpha-subunits appear
Valerie G Brunton et al.
Cancer research, 65(4), 1335-1342 (2005-03-01)
Src tyrosine kinase expression and activity are elevated during colon cancer progression. How this contributes to the malignant phenotype is not fully understood. We show that in KM12C colon carcinoma cells, expression of kinase-deficient Src proteins (SrcMF and Src251) does
R B Irby et al.
Oncogene, 19(49), 5636-5642 (2000-12-15)
Since the original identification of a transmissible agent responsible for the development of tumors in chickens, now known to be a retrovirus encoding the v-src gene, significant progress has been made in defining the potential functions of its human homolog
P L Schwartzberg
Oncogene, 17(11 Reviews), 1463-1468 (1998-10-21)
c-src was first isolated as the normal cellular homologue of v-src, the transforming gene of Rous Sarcoma virus (Stehelin et al., 1976). As the first proto-oncogene described and one of the first molecules demonstrated to have tyrosine kinase activity, Src
S M Thomas et al.
Annual review of cell and developmental biology, 13, 513-609 (1997-01-01)
Src family protein tyrosine kinases are activated following engagement of many different classes of cellular receptors and participate in signaling pathways that control a diverse spectrum of receptor-induced biological activities. While several of these kinases have evolved to play distinct

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