R8631
Bcl I from Bacillus caldolyticus
Restriction Enzyme
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
grade
for molecular biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
Specificity
Recognition sequence: 5′-T/GATCA-3′
Cutting results: a 2-10-fold Bcl I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: This enzyme cannot be heat inactivated at 65 °C for 15 minutes.
Cutting results: a 2-10-fold Bcl I overdigestion of 1 μg λ DNA substrate results in 100% cutting.
Heat inactivation: This enzyme cannot be heat inactivated at 65 °C for 15 minutes.
Application
BclI is a restriction endonuclease used in molecular biology applications to cut DNA at the recognition sequence 5′-T/GATCA-3′, resulting in fragments with 5′-cohesive termini.
Other Notes
Supplied with 10x Restriction Enzyme Buffer SM (B3158).
Cleavage activity: Bcl I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam+ strains).
Comment: Bcl I is optimally active at 50°C.
Comment: Bcl I is optimally active at 50°C.
Physical form
Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 200 mM NaCl, 10 mM 2-Mercaptoethanol, 0.2 % Triton X-100 (v/v), 50% glycerol (v/v) at 4°C
related product
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Bernd Kneidinger et al.
The Journal of biological chemistry, 278(6), 3615-3627 (2002-12-05)
N-Acetyl-l-fucosamine is a constituent of surface polysaccharide structures of Pseudomonas aeruginosa and Staphylococcus aureus. The three P. aeruginosa enzymes WbjB, WbjC, and WbjD, as well as the S. aureus homologs Cap5E, Cap5F, and Cap5G, involved in the biosynthesis of N-acetyl-l-fucosamine
A H Bingham et al.
Nucleic acids research, 5(10), 3457-3467 (1978-10-01)
The purification and characterization of a new restriction endonuclease, BclI from the extreme thermophile Bacillus caldolyticus is reported. This enzyme recognizes the sequence : formula: (see text) and cleaves at the positions indicated by the arrows.
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Rachel M Smith et al.
Nucleic acids research, 41(1), 391-404 (2012-11-14)
Type IIB restriction-modification systems, such as BcgI, feature a single protein with both endonuclease and methyltransferase activities. Type IIB nucleases require two recognition sites and cut both strands on both sides of their unmodified sites. BcgI cuts all eight target
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service