R8256
Sfi I from Streptomyces fimbriatus
Restriction Enzyme
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About This Item
Recommended Products
grade
Molecular Biology
for molecular biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Specificity
Recognition sequence: 5′-GGCC(N)4/NGGCC-3′
Cutting results: a 2-10-fold Sfi I overdigestion of 1 μg Ad-2 DNA substrate results in 100% cutting
Cutting results: a 2-10-fold Sfi I overdigestion of 1 μg Ad-2 DNA substrate results in 100% cutting
Application
SfiI is a DNA restriction enzyme that is used in molecular biological methods to cleave DNA at the recognition site 5′-GGCC(N)4/NGGCC-3′ to generate fragments with 3′-cohesive termini.
Other Notes
Supplied with 10x Restriction Enzyme Buffer SM (B3158).
Physical form
Solution in 10 mM Tris-HCl, pH 7.4, 0.1 mM EDTA, 5 mM MgCl2, 300 mM KCl, 7 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.2% Triton X-100 (v/v), 500 μg/ml BSA at 25 °C
related product
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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J M Johnson et al.
Applied and environmental microbiology, 61(7), 2806-2808 (1995-07-01)
Food and patient isolates from an Escherichia coli O157:H7 outbreak associated with undercooked ground beef were characterized by pulsed-field gel electrophoresis and Shiga-like toxin genotype. Pulsed-field gel electrophoresis confirmed the epidemiologically implicated source of the two-state outbreak and differentiated between
M Van Looveren et al.
Journal of clinical microbiology, 36(10), 2828-2834 (1998-09-17)
In Belgium an increase in the incidence of meningococcal disease has been noted since the early 1990s. Four hundred twenty clinical strains isolated during the period from 1990 to 1995, along with a set of 30 European reference strains, and
B Q Qiang et al.
Nucleic acids research, 12(11), 4507-4516 (1984-06-11)
A new site-specific endonuclease, Sfi I, has been isolated from Streptomyces fimbriatus . This is the first report of a type II restriction endonuclease whose recognition specificity requires eight nucleotides. Sfi I cleaves the sequence, GGCCNNNN / NGGCC , symmetrically
C Kessler et al.
Gene, 47(1), 1-153 (1986-01-01)
The properties and sources of all known restriction endonucleases and methylases are listed. The enzymes are cross-indexed (Table I), classified according to their recognition sequence homologies (Table II), and characterized within Table II by the cleavage and methylation positions, the
Rachel M Smith et al.
Nucleic acids research, 41(1), 391-404 (2012-11-14)
Type IIB restriction-modification systems, such as BcgI, feature a single protein with both endonuclease and methyltransferase activities. Type IIB nucleases require two recognition sites and cut both strands on both sides of their unmodified sites. BcgI cuts all eight target
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