grade
Molecular Biology
for molecular biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Specificity
Recognition sequence: 5′-ATGCA/T-3′
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Application
NsiI is a restriction endonuclease used in molecular biology to cleave DNA at the recognition site 5′-ATGCA/T-3′, generating DNA fragments with 3′-cohesive ends.
Other Notes
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Linkage
Isoschizomer: Ava III
Physical form
Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50 % glycerol (v/v), 0.02% polydocanol (v/v), 0.01% gelatine (v/v), at 4 °C
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Daniel Wegmüller et al.
Stem cells (Dayton, Ohio), 25(5), 1178-1185 (2007-01-16)
Although differentiation of pluripotent embryonic stem cells is restricted by a hierarchy of transcription factors, little is known about whether post-transcriptional mechanisms similarly regulate early embryoid differentiation. We developed a system where small hairpin (sh)RNAs can be induced in embryonic
Cloned NsiI restriction-modification system.
Longo, M.C., and Smith, M.D.
Biotechnology Advances, 15, 83-83 (1997)
T H Ch'ng et al.
Journal of virology, 79(14), 8835-8846 (2005-07-05)
Pseudorabies virus (PRV) glycoprotein E (gE) is a type I viral membrane protein that facilitates the anterograde spread of viral infection from the peripheral nervous system to the brain. In animal models, a gE-null mutant infection spreads inefficiently from presynaptic
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Rachel M Smith et al.
Nucleic acids research, 41(1), 391-404 (2012-11-14)
Type IIB restriction-modification systems, such as BcgI, feature a single protein with both endonuclease and methyltransferase activities. Type IIB nucleases require two recognition sites and cut both strands on both sides of their unmodified sites. BcgI cuts all eight target
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