grade
for molecular biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Specificity
Recognition sequence: 5′-ATGCA/T-3′
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Application
NsiI is a restriction endonuclease used in molecular biology to cleave DNA at the recognition site 5′-ATGCA/T-3′, generating DNA fragments with 3′-cohesive ends.
Other Notes
Supplied with 10x Restriction Enzyme Buffer SH (B3657).
Linkage
Isoschizomer: Ava III
Physical form
Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50 % glycerol (v/v), 0.02% polydocanol (v/v), 0.01% gelatine (v/v), at 4 °C
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Cloned NsiI restriction-modification system.
Longo, M.C., and Smith, M.D.
Biotechnology Advances, 15, 83-83 (1997)
T H Ch'ng et al.
Journal of virology, 79(14), 8835-8846 (2005-07-05)
Pseudorabies virus (PRV) glycoprotein E (gE) is a type I viral membrane protein that facilitates the anterograde spread of viral infection from the peripheral nervous system to the brain. In animal models, a gE-null mutant infection spreads inefficiently from presynaptic
Daniel Wegmüller et al.
Stem cells (Dayton, Ohio), 25(5), 1178-1185 (2007-01-16)
Although differentiation of pluripotent embryonic stem cells is restricted by a hierarchy of transcription factors, little is known about whether post-transcriptional mechanisms similarly regulate early embryoid differentiation. We developed a system where small hairpin (sh)RNAs can be induced in embryonic
Saqib H Ansari et al.
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
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