R2779
Renin human
BioUltra, recombinant, expressed in mammalian cells, ≥90 unit/μg protein, ≥98% (SDS-PAGE)
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About This Item
Application
Human renin is a member of the Peptidase A1 family. It catalyzes the hydrolysis of angiotensinogen to produce angiotensin I. It is used to study human renin-dependent hypertension as well as renin induced obesity .
Biochem/physiol Actions
Renin is a 406 amino acid precursor (45,057 Da) containing a 23 amino acid signal peptide and a 43 amino acid propeptide. Native active renin is a 340 amino acid glycoprotein containing three disulfide bonds. Renin, an aspartyl protease, cleaves angiotensinogen which results in Ang I. Ang I is converted to Ang II by the Ang-converting enzyme (ACE) . Prorenin is hydrolyzed by plasma kallekrein to produce active renin. The human renin transgene is thought to be responsible for increased appetite, obesity, and metabolic changes which are not related to angiotensin II .
Physical properties
Renin is processed as a 406 amino acid precursor (45,057 Da) containing a 23 amino acid signal peptide and a 43 amino acid propeptide. Native active renin is a 340 amino acid glycoprotein containing three disulfide bonds.
Supplied as a solution in 50 mM Tris HCl, pH 8
Unit Definition
One unit will produce one fluorescent unit from the hydrolysis of Renin Substrate I (Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys(dabcyl)-Arg) at 37 °C at pH 8.0
Preparation Note
Expressed in mammalian cells as prorenin. The prorenin is activated to renin using trypsin and re-purified.
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Manne Krop et al.
Hypertension (Dallas, Tex. : 1979), 61(5), 1075-1082 (2013-03-06)
Renin inhibitors like aliskiren not only block renin but also bind prorenin, thereby inducing a conformational change (like the change induced by acid) allowing its recognition in a renin-specific assay. Consequently, aliskiren can be used to measure prorenin. VTP-27999 is
Stephen J Nicholls et al.
JAMA, 310(11), 1135-1144 (2013-09-04)
Blood pressure reduction and renin-angiotensin-aldosterone system inhibition are targets for treatment of atherosclerosis. The effect of renin inhibition on coronary disease progression has not been investigated. To determine the effects of renin inhibition with aliskiren on progression of coronary atherosclerosis.
Brian G Keevil
Best practice & research. Clinical endocrinology & metabolism, 27(5), 663-674 (2013-10-08)
Liquid chromatography tandem mass spectrometry (LC-MS/MS) is increasingly becoming the method of choice for steroid hormone measurements due to small sample volumes, fast analysis times and improved specificity compared to immunoassays. Achievement of demanding analytical targets for steroid analysis is
Mihai Gheorghiade et al.
JAMA, 309(11), 1125-1135 (2013-03-13)
Hospitalizations for heart failure (HHF) represent a major health burden, with high rates of early postdischarge rehospitalization and mortality. To investigate whether aliskiren, a direct renin inhibitor, when added to standard therapy, would reduce the rate of cardiovascular (CV) death
Aldo P Maggioni et al.
European heart journal, 34(40), 3117-3127 (2013-09-04)
The objective of the Aliskiren Trial on Acute Heart Failure Outcomes (ASTRONAUT) was to determine whether aliskiren, a direct renin inhibitor, would improve post-discharge outcomes in patients with hospitalization for heart failure (HHF) with reduced ejection fraction. Pre-specified subgroup analyses
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