R1885
Swa I from Staphylococcus warneri
buffered aqueous glycerol solution
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Specificity
Recognition sequence: 5′-ATTT/AAAT-3′
Ligation and recutting results: After 2-10-fold Swa I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Ligation and recutting results: After 2-10-fold Swa I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Inactivated at 65 °C for 20 minutes.
Other Notes
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
Physical form
Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 500 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v) at 4 °C
Certificates of Analysis (COA)
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K Mise et al.
Gene, 33(3), 357-361 (1985-01-01)
A new restriction endonuclease, StyI, free of contaminating nuclease activities, has been isolated from Escherichia coli carrying the hsd+ miniplasmid of Salmonella typhi origin. In the presence of 10 mM Mg2+, it recognizes and cleaves a hexanucleotide sequence of 5'-C
Yu-Feng Huang et al.
BMC systems biology, 6 Suppl 2, S10-S10 (2013-01-11)
Current next-generation sequencing (NGS) platforms adopt two types of sequencing mechanisms: by synthesis or by ligation. The former is employed by 454 and Solexa systems, while the latter by SOLiD system. Although the pros and cons for each sequencing mechanism
Rachel M Smith et al.
Nucleic acids research, 41(1), 391-404 (2012-11-14)
Type IIB restriction-modification systems, such as BcgI, feature a single protein with both endonuclease and methyltransferase activities. Type IIB nucleases require two recognition sites and cut both strands on both sides of their unmodified sites. BcgI cuts all eight target
Nico Mitro et al.
Methods in molecular biology (Clifton, N.J.), 952, 137-144 (2012-10-27)
The role of certain amino acids in the interactions of ligands with their cognate nuclear receptors is usually achieved by the resolution of the crystal structure of the receptor complexed with the ligand. As a complementary functional approach, site-directed mutagenesis
D S Jansson et al.
British poultry science, 53(6), 790-799 (2013-02-13)
1. Vancomycin-resistant Enterococcus faecium (VRE(fm)) has recently spread among Swedish broiler farms. The objectives were to investigate VRE(fm) persistence within barns between flocks, and to determine whether day-old chicks, feed or forklift trucks used for loading crates could be identified
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