Anti-PTEN is developed in rabbit using a synthetic corresponding to the C-terminal of rat PTEN. Phosphatase and tensin homolog deleted on chromosome ten (PTEN), also called mutated in multiple advanced cancers (MMAC) codes for a 403 amino acid protein that is described as a dual-specificity protein phosphatase. However, considerable evidence indicates that PTEN is a lipid phosphatase. It has a N-terminal phosphatase domain with the catalytic core motif and a C-terminal PDZ binding domain. It is mainly localized to the cytoplasm. PTEN gene is located on human chromosome 10q23.31.
Specificity
Anti-PTEN recognizes rat PTEN (56 kDa).
Immunogen
synthetic peptide corresponding to amino acids 386-403 of mouse PTEN (also known as MMAC1 or TEP-1 tumor suppressor). This sequence is identical in rat PTEN and is highly conserved in human PTEN (single amino acid substitution).
Application
Anti-PTEN antibody produced in rabbit may be used in immunoblotting.
Biochem/physiol Actions
Phosphatase and tensin homolog deleted on chromosome ten (PTEN), a tumor suppressor gene is implicated in glioblastomas, endometrial carcinomas, advanced prostate cancers and breast cancers. In addition, germline mutations in PTEN give rise to Cowden disease, an autosomal-dominant syndrome, characterized by multiple hamartomas and increased risk for development of breast, thyroid and brain tumors. PTEN is a major negative regulator of the PI3-kinase-protein kinase B (PKB or Akt) signaling pathway, controlling cell proliferation and survival. Cells lacking functional PTEN, exhibit decreased sensitivity to cell death in response to a variety of apoptotic stimuli, accompanied by constitutively elevated activity of Akt/PKB.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Storage and Stability
For continuous use, store at 2-8 °C for up to one month.For extended storage, freeze in working aliquots.Repeated freezing and thawing is not recommended.Storage in "frost-free" freezers is not recommended. Ifslight turbidity occurs upon prolonged storage, clarifythe solution by centrifugation before use. Workingdilution samples should be discarded if not used within12 hours.
Disclaimer
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Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
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