N2509
2-Nitrophenyl 1-thio-β-D-galactopyranoside
for detection of lac mutants
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About This Item
Empirical Formula (Hill Notation):
C12H15NO7S
CAS Number:
Molecular Weight:
317.32
EC Number:
MDL number:
UNSPSC Code:
12352103
PubChem Substance ID:
Recommended Products
grade
for molecular biology
form
powder
storage temp.
−20°C
SMILES string
OC[C@H]1O[C@@H](Sc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O
InChI
1S/C12H15NO7S/c14-5-7-9(15)10(16)11(17)12(20-7)21-8-4-2-1-3-6(8)13(18)19/h1-4,7,9-12,14-17H,5H2/t7-,9+,10+,11-,12+/m1/s1
InChI key
SZAOZNVCHHBUDZ-RUXWNWLUSA-N
General description
2-Nitrophenyl 1-thio-β-D-galactopyranoside is an enzyme substrate used in the detection of lac- mutants.
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Ausubel, F.M., et al.
Current Protocols in Molecular Biology, 1-1 (1998)
S Gharbi et al.
Biochimie, 68(10-11), 1159-1163 (1986-10-01)
Mutants altered in their expression of the hybrid pepN-lacZ gene have been selected for resistance to p-nitrophenyl-beta-D-thiogalactopyranoside (a bacteriostatic compound that enters the cells via lac permease). A unique mutation decreasing the level of pepN expression to 9% of that
D S Gregerson et al.
Journal of immunology (Baltimore, Md. : 1950), 163(2), 1073-1080 (1999-07-08)
Recent studies revealing active mechanisms of immune privilege in neural tissues have diminished the putative role of passive tolerance. To examine the significance of Ag localization in the retina on immune privilege, the immune responses of transgenic mice expressing high
M T Martins et al.
Applied and environmental microbiology, 58(9), 3095-3100 (1992-09-01)
A total of 449 Escherichia coli isolates in treated and raw water sources were submitted to DNA-DNA hybridization using seven different DNA probes to detect homology to sequences that code for Shiga-like toxins I and II; heat-stabile and heat-labile toxins
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