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N214

Sigma-Aldrich

Neutrophil Activating Peptide-2 human

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

NAP-2

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About This Item

MDL number:
MFCD00241917
UNSPSC Code:
51111800

biological source

human

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

0.1-0.3 μg/mL ED50 (cytochalasin B treated human neutrophils)
0.1-0.5 ng/mL ED50 (BaF/3 hCXCR-2 transfected cells)

mol wt

~7.6 kDa

storage condition

avoid repeated freeze/thaw cycles (Storage in "frost-free" freezers is not recommended)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P02775

storage temp.

−20°C

Gene Information

human ... PPBP(5473)

Biochem/physiol Actions

Neutrophil Activating Peptide-2, a member of the CXC chemokine family, chemoattracts and activates neutrophils and also binds CXCR-2. NAP-2 is proteolytically processed C-terminal fragments of platelet basic protein (PBP), which is found in the α-granules of human platelets.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by the induction of myeloperoxidase release from cytochalasin B treated human neutrophils.

Certificates of Analysis (COA)

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A E Proudfoot et al.
Journal of protein chemistry, 16(1), 37-49 (1997-01-01)
Connective tissue-activating peptide III (CTAP-III) and neutrophil-activating peptide-2 (NAP-2) are both derived from a common precursor, platelet basic protein (PBP), which is stored in the alpha-granules of platelets and released upon their activation. CTAP-III is an 85-residue peptide which is
N Iida et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 10(11), 1336-1345 (1996-09-01)
Leukocytes produce many biological mediators that orchestrate the subsequent cellular events during wound healing. We have identified a novel cytokine, leukocyte-derived growth factor (LDGF), which is mitogenic for connective tissue cells. Sequence analysis of the LDGF peptide revealed that it
J M Schröder et al.
Journal of immunology (Baltimore, Md. : 1950), 139(10), 3474-3483 (1987-11-15)
A novel monocyte-derived neutrophil-activating peptide (MONAP) produced by lipopolysaccharide- and phorbol myristate acetate-stimulated human peripheral blood monocytes was purified by sequential ion exchange-high performance liquid chromatography (HPLC), size exclusion HPLC, and reversed phase HPLC. Biologic activities of the purified cytokine

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