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M8410

Sigma-Aldrich

Mineral oil

light oil, (neat)

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5 ML
$19.50
100 ML
$38.90
500 ML
$63.80
1 L
$108.00

$19.50


Estimated to ship onMarch 29, 2025


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5 ML
$19.50
100 ML
$38.90
500 ML
$63.80
1 L
$108.00

About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

$19.50


Estimated to ship onMarch 29, 2025


Request a Bulk Order

Quality Level

sterility

non-sterile

form

light oil

storage condition

protect from light

refractive index

n20/D 1.467 (lit.)

density

0.84 g/mL at 25 °C (lit.)

format

neat

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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application

Mineral oil has been used as an overlay in microdrop embryo cultures.[1][2][3]

Caution

This product is not sterile. Mineral oil is difficult to sterilize. It can only be sterilized by careful filtration of the warmed oil. It is assumed antibiotics will be added to the cell culture medium. Otherwise, Prod. No. M5310 should be used, as it is sterile filtered.

Analysis Note

This material is tested as an overlay in microdrop embryo cultures. (All embryo tested materials are tested using mouse embryos.) Typically BCF1 x BDF1 hybrids are used. The oil is primarily used to prevent evaporation of the medium, particiularly when performing embryo manipulations.1 The procedure is as follows: 1-cell (BCF1 X BDF1) embryos were cultured in triplicate microdrops of embryo-tested HTF (Human Tubal Fluid) medium supplemented with 0.4% BSA. For a valid assay, at least 70% of 1-cell stage control embryos must develop to the blastocyst stage within 96 hours. For product release, at least 80% of 1-cell stage test embryos must develop to the blastocyst stage within 96 hours with the tested material.

comparable product

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

No data available

flash_point_c

No data available

ppe

Eyeshields, Gloves


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Certificates of Analysis (COA)

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TIE: A Method to Electroporate Long DNA Templates into Preimplantation Embryos for CRISPR-Cas9 Gene Editing
Bagheri H, et al.
The CRISPR journal, 1(3), 223-229 (2018)
Minoru Kubo et al.
Nucleic acids research, 47(9), 4539-4553 (2019-03-16)
Next-generation sequencing technologies have made it possible to carry out transcriptome analysis at the single-cell level. Single-cell RNA-sequencing (scRNA-seq) data provide insights into cellular dynamics, including intercellular heterogeneity as well as inter- and intra-cellular fluctuations in gene expression that cannot
Insulin and branched-chain amino acid depletion during mouse preimplantation embryo culture programmes body weight gain and raised blood pressure during early postnatal life
Velazquez M
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1864(2), 590-600 (2018)
Production of a gonadotropin-releasing hormone 2 receptor knockdown (GNRHR2 KD) swine line
Desaulniers AT, et al.
Transgenic research, 26(4), 567-575 (2017)
Takaya Totsuka et al.
Scientific reports, 10(1), 6556-6556 (2020-04-18)
To study the functional differences between maternal and paternal genomes in mammalian development, embryos with only one parental genome are often used. Androgenetic embryos are produced by the removal of maternal chromosomes before or after fertilization by techniques that require

Articles

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

Questions

1–4 of 4 Questions  
  1. Hello,I´m looking for a mineral oil for sulfur pattern production. It´s difficult to find an oil in which the content of sulfur compounds appears in the COA. Can you inform if there´s a possibility of sulfur contamination in the production of mineral oil?

    1 answer
    1. The manufacturing process for this product is considered proprietary. Currently, there is no product option available that is tested for the presence of sulfur.

      Helpful?

  2. ıs this suitable for preparation of carbon paste electrode?

    1 answer
    1. This material has not been validated in the process of making carbon paste electrodes. However, if the specifications (found below) meet your requirements, then there is no reason that they can't be used in this process.
      https://www.sigmaaldrich.com/specification-sheets/990/164/M8410-BULK.pdf

      Helpful?

  3. Hello, Does this product have an odour? I want to use it for an olfactory memory assay, so needs to be odourless

    1 answer
    1. According to Section 9 of the Safety Data Sheet, this is an odorless chemical.

      Helpful?

  4. What is the composition of the oil? Where does it comes from?

    1 answer
    1. M8410 is a light oil. Mineral oil is a complex mixture of different hydrocarbons and its typical composition is as follows:

      Paraffinic oils, based on n-alkanes, 15-60%
      Naphthenic oils, based on cycloalkanes, 30%-60%
      Aromatic oils, based on aromatic hydrocarbons, 3-30%
      Light and heavy mineral oil differ in terms of composition, viscosity, and density.
      Mineral oil with an alkyl chain carbon number of 15 to 25 is categorized as light oil whereas 25 to 50 as heavy oil.
      Light oil has a density of 0.83–0.86 kg L-1 whereas heavy oil has 0.875–0.905 kg L-1
      Light mineral oil is less occlusive than heavy mineral oil as it contains more cyclic (saturated) molecules

      Additional Links: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/326/294/m8410pis.pdf and a second document: https://www.sigmaaldrich.com/specification-sheets/990/164/M8410-BULK.pdf

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