immunoprecipitation (IP): 5 μg using 0.5-1 mg of a mouse 3T3 fibroblast lysate western blot: 0.5-2 μg/mL using human A431 cells, mouse 3T3 fibroblasts or rat L6 cells
Mitogen-activated protein kinases (MAPKs) regulate a wide range of functions by modulating cellular signal transduction. These functions include cellular growth, differentiation, movement and apoptosis. Conventional MAPKs comprise of the ERK1/2, p38, JNK and ERK5 kinase families. ERK1 and ERK2 are highly conserved serine/threonine kinases that are responsive to growth factors Anti-MAP Kinase 2 (ERK-2) antibody is specific for MAPK2 (42 kDa) in mice, humans and rats. This antibody does not cross react with MAP kinase 1 (ERK-1) nor does it interfere with the kinase activity.
Immunogen
recombinant mouse MAP Kinase 2 (MAPK2, ERK-2).
Application
Anti-MAP Kinase 2 (ERK-2) antibody is suitable for use in western blot (0.5-2 μg/mL using human A431 cells, mouse 3T3 fibroblasts or rat L6 cells). The product can also be used for immunoprecipitation (5 μg, using 0.5-1 mg of a mouse 3T3 fibroblast lysate).
Physical form
Solution in 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and ≤ 0.1% sodium azide
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Extracellular signal-regulated kinases 1 and 2 are growth factor-sensitive serine/threonine kinases. cDNAs for both human kinases were isolated and sequenced. The nucleic acid and deduced protein sequences of human extracellular signal-regulated kinase 1 were 88% and 96% identical, respectively, to
Cell chemical biology, 27(12), 1573-1581 (2020-10-03)
Here, we report a method to regulate cellular protein levels by introducing a ubiquitin variant between a destabilizing domain (DD) and the regulated protein. When produced in the absence of a stabilizing ligand the DD dominates and the entire fusion
Microbiology and molecular biology reviews : MMBR, 75(1), 50-83 (2011-03-05)
The mitogen-activated protein kinases (MAPKs) regulate diverse cellular programs by relaying extracellular signals to intracellular responses. In mammals, there are more than a dozen MAPK enzymes that coordinately regulate cell proliferation, differentiation, motility, and survival. The best known are the
The present study assessed the mechanism underlying the effect of sorafenib on the proliferation and apoptosis of the acute promyelocytic leukemia (APL) cell line NB4. NB4 cells were treated with different concentrations of sorafenib (0, 1.5, 3, 6, and 12
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