M251
Macrophage-Derived Chemokine human
>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
MDC, STCP-1
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
MDL number:
UNSPSC Code:
51111800
biological source
human
recombinant
expressed in E. coli
assay
>97% (SDS-PAGE)
form
lyophilized powder
potency
0.002-0.006 μg/mL ED50
mol wt
Mw ~8 kDa
storage condition
avoid repeated freeze/thaw cycles (Storage in "frost-free" freezers is not recommended)
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... CCL22(6367)
Biochem/physiol Actions
Macrophage-Derived Chemokine (MDC) induces chemotaxis or calcium mobilization in dendritic cells, IL-2 activated cells, and activated T lymphocytes. MDC, also named stimulated T cell chemotactic protein (STCP-1), shows <35% identity to other CC chemokine family members. It is expressed in dendritic cells, macrophages, and activated monocytes. Expression is also detected in the tissues of thymus, lymph node, and appendix.
Physical form
Lyophilized from a 0.2 μm filtered solution in 20% acetonitrile and 0.1% trifluoroacetic acid containing 1.25 mg bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to chemoattract human T-lymphoblastoid CEM-NKr cells.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
D N Howell et al.
Journal of immunology (Baltimore, Md. : 1950), 134(2), 971-976 (1985-02-01)
The human T lymphoblastoid cell line CEM was subjected to immunoselection by co-culture with peripheral blood mononuclear cells (PBMC) for resistance to natural killer (NK) cell-mediated lysis. The NK susceptibility of the resulting subline, CEM.NKR, was 8.4 to 20.6% of
R Godiska et al.
The Journal of experimental medicine, 185(9), 1595-1604 (1997-05-05)
A cDNA encoding a novel human chemokine was isolated by random sequencing of cDNA clones from human monocyte-derived macrophages. This protein has been termed macrophage-derived chemokine (MDC) because it appears to be synthesized specifically by cells of the macrophage lineage.
M s Chang et al.
The Journal of biological chemistry, 272(40), 25229-25237 (1997-10-06)
A novel human chemokine STCP-1 (stimulated T cell chemotactic protein) was isolated from an activated macrophage cDNA library. The chemokine has four cysteines positioned in a manner that identifies STCP-1 as a member of the CC chemokine family. The amino
R Pal et al.
Science (New York, N.Y.), 278(5338), 695-698 (1997-10-24)
CD8(+) T lymphocytes from individuals infected with human immunodeficiency virus-type 1 (HIV-1) secrete a soluble activity that suppresses infection by HIV-1. A protein associated with this activity was purified from the culture supernatant of an immortalized CD8(+) T cell clone
T Imai et al.
The Journal of biological chemistry, 273(3), 1764-1768 (1998-01-27)
Macrophage-derived chemokine (MDC) is a recently identified member of the CC chemokine family. MDC is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine (TARC), which contains 37% identical amino acids. Both chemokines are highly
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
