Leukemia inhibitory factor (LIF) is a pleotrophic glycoprotein that belongs to the IL6 family of cytokines. It is secreted by many cell types such as, fibroblasts, macrophages, T cells, monocytes, liver cells, osteoblasts and cells of endometrium. LIF interacts with a heterodimeric receptor composed of two subunits, gp130 and gp190. A variety of signal transduction pathways are triggered in response to LIF including ERK/MAPK, JAK1, PI3K, STAT3, Jun-cFos and NF-κB. The various functions of LIF include proliferation of adult human T cells, maintenance of pluripotency of embryonic stem cells and inhibition of leukemic ell differentiation. Reports also indicate the important role of LIF in lactation, tissue regeneration after brain and spinal cord injury, muscle regeneration and motor neuron functions. Anti-Leukemia Inhibitory Factor recognizes human leukemia inhibitory factor. It shows no cross-reactivity with recombinant mouse LIF.
Specificity
The antibody neutralizes the biological activity of recombinant human LIF.
Immunogen
recombinant human LIF expressed in E. coli
Application
Anti-Leukemia Inhibitory Factor antibody may be for immunoblotting a concentration of 1 μg/ml For ELISA a working concentration of 1 μg/ml is recommended. The antibody is suitable for neutralization reactions. For neutralization of LIF in SEK1, melanoma cell line, a concentration of 100 μg/ml was used.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline
We found that human melanoma SEKI and neuroepithelioma NAGAI cells, which are known to secrete high concentrations of leukemia inhibitory factor (LIF), also secrete high levels of hepatocyte growth factor (HGF). We therefore examined the role of LIF in HGF
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Leukemia inhibitory factor (LIF) is an important regulator of skeletal muscle regeneration and has been suggested to be mitogenic for myogenic cells because it has been shown to increase the quantity of myoblast cells grown in culture over extended periods
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