I152
I-309 human
>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
MDL number:
UNSPSC Code:
51111800
biological source
human
recombinant
expressed in E. coli
assay
>97% (SDS-PAGE)
form
lyophilized powder
potency
30-60 ng/mL ED50
mol wt
8.5 kDa
storage condition
avoid repeated freeze/thaw cycles (Storage in "frost-free" freezers is not recommended)
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
Storage temp.
−20°C
Gene Information
human ... CCL1(6346)
Biochem/physiol Actions
I-309, a chemoattractant for monocytes but not neutrophils, is an approximately 8.5 kDa polypeptide of 73 amino acids. Human I-309 is thought to be a homolog of the mouse TCA3, and shows 42% amino acid homology to mouse TCA3.
I-309, a glycoprotein, is a chemoattractant for monocytes.[1] It is secreted by monocytes, activated macrophages and T lymphocytes.[2] Presence of I-309 in lymph node lymphatic sinuses controls metastases into the lymph nodes.[3] It suppresses CCR8 (C-C motif chemokine receptor 8)-induced HIV (human immunodeficiency virus) infection and also participates in angiogenesis.[2] I-309 is also referred to as CCL1 (C-C motif chemokine ligand 1).
Physical form
Lyophilized from 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.
Analysis Note
Bioactivity measured by ability of I-309 to chemoattract hCCR8 transfected BaF/3 cells.
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Inhalation - Eye Irrit. 2
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Rita Crinelli et al.
Antioxidants (Basel, Switzerland), 10(2) (2021-02-04)
I-152 combines two pro-glutathione (GSH) molecules, namely N-acetyl-cysteine (NAC) and cysteamine (MEA), to improve their potency. The co-drug efficiently increases/replenishes GSH levels in vitro and in vivo; little is known about its mechanism of action. Here we demonstrate that I-152
Suvendu Das et al.
The Journal of experimental medicine, 210(8), 1509-1528 (2013-07-24)
Lymphatic vessels are thought to contribute to metastasis primarily by serving as a transportation system. It is widely believed that tumor cells enter lymph nodes passively by the flow of lymph. We demonstrate that lymph node lymphatic sinuses control tumor
M D Miller et al.
Journal of immunology (Baltimore, Md. : 1950), 143(9), 2907-2916 (1989-11-01)
We have identified two cDNA clones, I-309 and G-26, which define genes expressed abundantly in activated human PBMC, but at low or undetectable levels in resting PBMC. Based upon nucleotide sequence analysis, both clones are predicted to encode small, structurally
M D Miller et al.
Proceedings of the National Academy of Sciences of the United States of America, 89(7), 2950-2954 (1992-04-01)
The human cytokine I-309 is a small glycoprotein secreted by activated T lymphocytes and structurally related to a number of inflammatory cytokines. To investigate the biological activities of I-309 protein, we produced a stable Chinese hamster ovary cell transfectant, CDI.10
Catherine Denis et al.
PloS one, 7(3), e34199-e34199 (2012-04-06)
Carboxypeptidase M (CPM) targets the basic amino acids arginine and lysine present at the C-terminus of peptides or proteins. CPM is thought to be involved in inflammatory processes. This is corroborated by CPM-mediated trimming and modulation of inflammatory factors, and
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
