G1663
Glycogen Synthase Kinase 3β, Histidine-tagged from rabbit
≥90% (SDS-PAGE), recombinant, expressed in E. coli
Synonym(s):
GSK-3β, Kinase FA, Tau protein kinase I
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About This Item
recombinant
expressed in E. coli
assay
≥90% (SDS-PAGE)
form
lyophilized solid
specific activity
5000.00-30000.00 units/mg protein
mol wt
46,712 Da
relevant disease(s)
Alzheimer′s disease
shipped in
dry ice
storage temp.
−20°C
General description
Glycogen Synthase Kinase 3β (GSK3β) is one of the two isoforms of GSK3 enzyme, both of which are encoded by different genes. This protein is predominantly cytoplasmic, but is also found to be localized to nucleus and mitochondria. It is a protein-serine kinase. This protein contains a protein kinase domain in its core, and is composed of 482 amino acids with a molecular weight of 46,712Da. It shows a wide range of tissue expression with the highest expression in brain.
Application
Glycogen Synthase Kinase 3β (GSK3β) has been used
- to study GSK3β cleavage by calpain
- to perform motility assay on squid axoplasms
- for the pre-incubation of progesterone receptor A during in vitro ubiquitination assay
Biochem/physiol Actions
Glycogen Synthase Kinase 3β (GSK3β) is responsible for rapid cyclin D1 turnover by phosphorylating it on Thr-286. It functions as a negative regulator of Wnt signaling during embryonic development cell proliferation in adult tissues. This protein phosphorylates and regulates multiple proteins, such as glycogen synthase, insulin receptor substrate-1, axin and APC (adenomatous polyposis coli). This protein is also shown to interact with multiple proteins, which are linked with Alzheimer′s disease (AD).
Unit Definition
One unit will transfer one pmole of phosphate from ATP to phosphatase inhibitor 2 per min at pH 7.5 at 30 °C.
Reconstitution
Reconstitution to 100 μl with deionized water produces a solution in 20 mM Tris, pH 7.5, 2 mM EDTA, 2 mM EGTA, 1 mM DTT, 5% sucrose, 0.1% Brij® L23, 1 μg/ml leupeptin, and 0.1 mM AEBSF.
Legal Information
Brij is a registered trademark of Croda International PLC
inhibitor
Product No.
Description
Pricing
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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Shaohui Wang et al.
The Journal of biological chemistry, 288(36), 26265-26274 (2013-07-25)
Germ line mutations of the BRCA1 gene increase the risk of breast and ovarian cancer, but the basis of this tissue-specific tumor predisposition is not fully understood. Previously, we reported that the progesterone receptors are stabilized in Brca1-deficient mammary epithelial
Cohen, P.
The Enzymes, 17, 461-461 (1986)
K Hughes et al.
The Biochemical journal, 288 ( Pt 1), 309-314 (1992-11-15)
Multifunctional ATP-citrate lyase kinase (ACLK) exhibits several properties that are similar to glycogen-synthase kinase-3 (GSK-3). The molecular cloning of two distinct mammalian GSK-3 cDNAs and a Drosophila melanogaster (fruitfly) homologue, zeste-white3sgg, has established the existence of a GSK-3 subfamily. A
J S Song et al.
Journal of protein chemistry, 14(2), 95-105 (1995-02-01)
Previously, tau protein kinase I/glycogen synthase kinase-3 beta/kinase FA(TPKI/GSK-3 beta/FA) was identified as a brain microtubule-associated tau kinase possibly involved in the Alzheimer disease-like phosphorylation of tau. In this report, we find that the TPKI/GSK-3 beta/FA can be stimulated to
Paloma Goñi-Oliver et al.
The Journal of biological chemistry, 282(31), 22406-22413 (2007-06-16)
Although GSK-3 activity can be regulated by phosphorylation and through interaction with GSK-3-binding proteins, here we describe N-terminal proteolysis as a novel way to regulate GSK-3. When brain extracts were exposed to calcium, GSK-3 was truncated, generating two fragments of
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