form
lyophilized powder
usage
sufficient for 100 tests
storage temp.
2-8°C
Application
The preparation contains a receptor binding protein in a stable, lyophilized form convenient to use for a radioreceptor assay (RRA) for benzodiazepines. The method is based on the competition for specific receptors between biologically active benzodiazepine in biological fluids and 3H-flunitrazepam. The specificity of the receptor preparation is similar to that of fresh membrane-bound rat brain benzodiazepine receptors. Lorazepam is used as a standard unless a specific benzodiazepne is preferred.
Reconstitution
400 mg receptor preparation (the contents of one vial) is suspended in 40 mL sodium phosphate buffer on the day of the assay. The suspension is stable for one day at RT and for more than 8 months at -80 °C. It can be thawed and refrozen several times during this period.
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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J Lund
Scandinavian journal of clinical and laboratory investigation, 41(3), 275-280 (1981-05-01)
A method for determination of benzodiazepines in human blood, plasma, saliva and urine has been developed. The method is based upon the competition between 3H-flunitrazepam and biologically active benzodiazepines in biological fluids for brain specific receptors, prepared in a stable
R G Dorow et al.
British journal of clinical pharmacology, 13(4), 561-565 (1982-04-01)
1 A radioreceptor assay has been established to measure the receptor affinities of numerous benzodiazepines in clinical use. 2 The time course of receptor binding activity was studied by this method in the plasma of eight healthy subjects randomly treated
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