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D7690

Sigma-Aldrich

Monoclonal Anti-DNA Ligase I antibody produced in mouse

~1 mg/mL, clone 1A9, purified immunoglobulin, buffered aqueous solution

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About This Item

MDL number:
MFCD02686095
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1A9, monoclonal

form

buffered aqueous solution

mol wt

antigen ~125 kDa

species reactivity

bovine, human

concentration

~1 mg/mL

technique(s)

direct ELISA: suitable
western blot: 1-5 μg/mL using HeLa or DiFi cells

isotype

IgG1

UniProt accession no.

P18858

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... LIG1(3978)

General description

DNA ligase I is composed of a 78kDa carboxyl-terminal catalytic domain and a 24kDa amino-terminal region.

Immunogen

bovine DNA ligase I.

Application

Monoclonal Anti-DNA Ligase I antibody produced in mouse is suitable for direct ELISA and western blot at a working concentration of 1-5μg/mL using HeLa or DiFi cells.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 0.08% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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V J Mackenney et al.
The Journal of biological chemistry, 272(17), 11550-11556 (1997-04-25)
The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is believed to be due to DNA ligase I. This enzyme is composed of a 78-kDa carboxyl-terminal catalytic domain and a 24-kDa amino-terminal region that is not

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