Skip to Content
MilliporeSigma
All Photos(6)

Key Documents

View All Documentation

C0875

Sigma-Aldrich

Calcein

Powder

Synonym(s):

Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein, Fluorescein-bis(methyliminodiacetic acid), Fluorexon

Sign Into View Organizational & Contract Pricing
All Photos(6)

About This Item

Empirical Formula (Hill Notation):
C30H26N2O13
CAS Number:
154071-48-4
Molecular Weight:
622.53
Beilstein/REAXYS Number:
8181068
UNSPSC Code:
12171500
PubChem Substance ID:
24892279
NACRES:
NA.47

product name

Calcein, Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.

Quality Level

200

description

mixed isomers

form

powder

technique(s)

titration: suitable

color

yellow to brown

solubility

NaOH: 50 mg/mL

fluorescence

λex 470 nm; λem 509 nm (pH 7.4)

suitability

suitable for (Suitable for fluorometric determination of Ca Suitable for EDTA titration of Ca in the presence of Mg)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OC(=O)CN(CC(O)=O)Cc1cc2c(Oc3cc(O)c(CN(CC(O)=O)CC(O)=O)cc3C24OC(=O)c5ccccc45)cc1O

Looking for similar products? Visit Product Comparison Guide

Application

Calcein has been used:
  • in whole cell staining
  • in histomorphometry and micro-computed tomography
  • to label active bone-forming surfaces

Biochem/physiol Actions

Calcein is a calcium-dependent fluorescent molecule. It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions. Calcein is used for the fluorometric determination of calcium and EDTA (ethylenediaminetetraacetic acid) titration of calcium.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Parathyroid hormone-related peptide is required for increased trabecular bone volume in parathyroid hormone-null mice.
Miao D, et al.
Endocrinology, 145, 3554-3554 (2004)
Kengo Sato et al.
Clinical science (London, England : 1979), 133(16), 1779-1796 (2019-08-11)
Plasma levels of chemerin, an adipocytokine produced from the adipose tissues and liver, are associated with metabolic syndrome and coronary artery disease (CAD). Chemerin and its analog, chemerin-9, are known to bind to their receptor, ChemR23. However, whether chemerin and
Ragnhild Haugse et al.
Pharmaceutics, 11(7) (2019-07-10)
Treatment with ultrasound and microbubbles (sonoporation) to enhance therapeutic efficacy in cancer therapy is rapidly expanding, but there is still very little consensus as to why it works. Despite the original assumption that pore formation in the cell membrane is
Wnt10b deficiency results in age-dependent loss of bone mass and progressive reduction of mesenchymal progenitor cells
Stevens JR, et al.
Journal of Bone and Mineral Research, 25(10), 2138-2147 (2010)
Skeletal abnormalities in Pth-null mice are influenced by dietary calcium.
Miao D, et al.
Endocrinology, 145, 2046-2046 (2004)
View All Related Papers

Articles

Sensor Dyes

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service