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A3809

Sigma-Aldrich

Asparaginase from Escherichia coli

lyophilized powder, 100-300 units/mg protein (biuret)

Synonym(s):

L-Asparagine Amidohydrolase

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About This Item

CAS Number:
9015-68-3
Enzyme Commission number:
3.5.1.1 (BRENDA, IUBMB)
EC Number:
232-765-3
MDL number:
MFCD00130565
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

200

specific activity

100-300 units/mg protein (biuret)

composition

Protein, ≥60%

storage temp.

2-8°C

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General description

Asparaginase is a bacterial enzyme and also a chemotherapeutic drug.

Application

Asparaginase from Escherichia coli has been used:
  • to compare the cytotoxic effect of L-asparaginase purified from Streptomyces brollosae NEAE-115
  • as a standard in asparaginase assay to quantify asparaginase activities in various eel tissues
  • to elicit amino acid deprivation

Biochem/physiol Actions

Asparaginase (ASNase) products are usually obtained from Escherichia coli and Erwinia chrysanthemi. These enzymes can block the synthesis of protein in tumor cells. It shows high activity in the G1 phase of the cell cycle. It is capable of causing pancreatitis in leukemia patients.
Asparaginase is used in enzymatic assays and to convert asparagine to aspartic acid. Asparaginase is used to reduce the formation of acrylamide in starchy food products. It is also used as a chemotherapy agent for acute lymphoblastic leukemia . Product A3809 is from Escherichia coli and is provided as a lyophilized powder containing sodium chloride.
Asparaginase may cause cell death in leukemic cells by converting the necessary L-asparagine to aspartic acid and ammonia . Asparaginase is allosterically regulated and is crucial for proper cell functioning .

Unit Definition

One unit will liberate 1.0 μmole of ammonia from L-asparagine per min at pH 8.6 at 37 °C.

Physical form

Lyophilized powder containing sodium chloride

Preparation Note

Chromatographically purified

Analysis Note

Protein determined by biuret.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

hcodes

H317,H361

Hazard Classifications

Repr. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jie Xiong et al.
EBioMedicine, 72, 103614-103614 (2021-10-11)
Metabolic reprogramming plays an essential role on lymphoma progression. Dysregulation of glutamine metabolism is implicated in natural-killer T-cell lymphoma (NKTCL) and tumor cell response to asparaginase-based anti-metabolic treatment. To understand the metabolomic alterations and determine the potential therapeutic target of
Asparagine Synthetase Is Highly Expressed at Baseline in the Pancreas Through Heightened PERK Signaling
Mukherjee A, et al.
Cellular and molecular gastroenterology and hepatology (2019)
Purification, characterization and immunogenicity assessment of glutaminase free L-asparaginase from Streptomyces brollosae NEAE-115
El-Naggar NEA, et al.
BMC Pharmacology & Toxicology, 19(1), 1-15 (2018)
Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells
Hitzel J, et al.
Nature Communications, 9(2292), 1-18 (2018)
L-Asparaginase: discovery and development as a tumor-inhibitory agent.
J D Broome
Cancer treatment reports, 65 Suppl 4, 111-114 (1981-01-01)
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