A1731
Aminophenyl fluorescein
Synonym(s):
2-[6-(4′-Amino)phenoxy-3H-xanthen-3-on-9-yl] benzoic acid, APF
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About This Item
Empirical Formula (Hill Notation):
C26H17NO5
Molecular Weight:
423.42
UNSPSC Code:
12352200
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storage condition
protect from light
fluorescence
λex 490 nm; λem 515 nm
storage temp.
2-8°C
Application
Fluorescent detector of highly reactive oxygen species (hROS). Fluorescence intensity increases after reaction with hROS (e.g. hydroxy radical, peroxynitrite and hypochlorite). Since APF does not react with nitric oxide, superoxide, and hydrogen peroxide, it can be used to differentially detect peroxynitrite. APF is resistant to light-induced autoxidation.
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