V900483

Trizma^® base

≥99%, crystalline powder, reagent grade, Vetec^™

• Synonym(s): 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
• Linear Formula: NH₂C(CH₂OH)₃
• CAS Number: 77-86-1
• Molecular Weight: 121.14
• Beilstein/REAXYS Number: 741883
• EC Number: 201-064-4
• MDL number: MFCD00004679
• UNSPSC Code: 12161700
• PubChem Substance ID: 329829884

Properties

• product name: Trizma^® base, Vetec^™, reagent grade, ≥99%
• grade: reagent grade
• description: aminopeptidase substrate
• product line: Vetec^™
• assay: ≥99%
• form: crystalline powder
• pH: 10.5-12
• useful pH range: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• solubility: water: 0.667 g/mL, clear, colorless to faintly yellow
• SMILES string: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

Trizma^® base is a basimetric standard used in biochemistry and molecular biology. It may be used as a buffer or as a component of mixed buffer formulations. It is essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Trizma^® base can be used as a buffer:

• In the evanescent absorption analysis of NADPH.
• In the autoxidation studies of oxymyoglobin.

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Vetec is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable